概述

  • 产品名称Anti-ELAVL4抗体
    参阅全部 ELAVL4 一抗
  • 描述
    兔多克隆抗体to ELAVL4
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Rabbit, Chicken, Human, Macaque Monkey, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse ELAVL4.

  • 阳性对照
    • This antibody gave a positive signal within Western Blot in the following tissue lysates: Mouse Brain P7; Rat Brain P7; Mouse Brain P0; Rat Brain P0. This antibody gave a positive result in IHC in the following FFPE tissue: Human hippocampus.

性能

应用

Our Abpromise guarantee covers the use of ab96474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Abcam recommends using milk as the blocking agent (3%).

靶标

  • 功能May play a role in neuron-specific RNA processing. Protects CDKN1A mRNA from decay by binding to its 3'-UTR (By similarity). Binds to AU-rich sequences (AREs) of target mRNAs, including VEGF and FOS mRNA.
  • 组织特异性Brain.
  • 序列相似性Belongs to the RRM elav family.
    Contains 3 RRM (RNA recognition motif) domains.
  • 翻译后修饰Methylation at Arg-243 by CARM1 weakens protective binding to the 3'-UTR of CDKN1A mRNA and down-regulates CDKN1A protein expression, thereby maintaining cells in a proliferative state. Methylation is inhibited by NGF, which facilitates neurite outgrowth.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ELAV (embryonic lethal abnormal vision Drosophila) like 4 antibody
    • ELAV L4 antibody
    • ELAV like 4 antibody
    • ELAV like protein 4 antibody
    • ELAV-like protein 4 antibody
    • ELAV4_HUMAN antibody
    • Elavl4 antibody
    • Embryonic lethal abnormal vision Drosophila homolog of like 4 antibody
    • Hu antigen D antibody
    • Hu-antigen D antibody
    • HuD antibody
    • Paraneoplastic encephalomyelitis antigen HuD antibody
    • PNEM antibody
    see all

Anti-ELAVL4 antibody 图像

  • ICC/IF image of ab96474 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96474 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-ELAVL4 antibody (ab96474) at 1 µg/ml (Milk blocking - 3%)

    Lane 1 : P7 Mouse Brain Tissue Lysate Lab Lysate
    Lane 2 : P7 Rat Brain Rat Tissue Lysate
    Lane 3 : P0 Mouse Brain Mouse Tissue Lysate
    Lane 4 : P0 Rat Brain Rat Tissue Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 40,42 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes
  • IHC image of ELAVL4 staining in Human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96474, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-ELAVL4 antibody (ab96474)参考文献

ab96474 has not yet been referenced specifically in any publications.

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