Anti-ELAVL2 + ELAVL4抗体(ab72603)


  • 产品名称Anti-ELAVL2 + ELAVL4抗体
    参阅全部 ELAVL2 + ELAVL4 一抗
  • 描述
    兔多克隆抗体to ELAVL2 + ELAVL4
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from an internal region of human ELAVL2 + ELAVL4.

  • 阳性对照
    • Extracts from HUVEC cells and Jurkat cells. HepG2 cells.



Our Abpromise guarantee covers the use of ab72603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明ELISA: 1/10000.
    ICC/IF: 1/500 - 1/1000.
    WB: 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weights: 40 and 42 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性ELAV2 binds RNA and seems to recognize a GAAA motif and is neuronal specific. ELAVL4 may play a role in neuron-specific RNA processing. It contains 3 RNA recognition motif (RRM) domains and is localised to brain tissue. ELAVL4 has been linked with the disease, paraneoplastic encephalomyelitis sensory neuropathy, which is associated with small cell lung cancer and is characterized by dementia, sensory loss, and other neurological disabilities. This disease may result from an immune response primarily directed against a small cell lung tumor antigen which is misdirected against similar antigens expressed in brain (such as ELAVL4).
    • 数据库链接
    • 别名
      • ELAV like 4 antibody
      • ELAV like neuronal protein 1 antibody
      • ELAV like protein 2 antibody
      • ELAV like protein 4 antibody
      • ELAVL 2 antibody
      • ELAVL 4 antibody
      • HELN1 antibody
      • Hu antigen B antibody
      • Hu antigen D antibody
      • HUB antibody
      • HUD antibody
      see all

    Anti-ELAVL2 + ELAVL4 antibody 图像

    • All lanes : Anti-ELAVL2 + ELAVL4 antibody (ab72603) at 1/500 dilution

      Lane 1 : Extracts from HUVEC cells
      Lane 2 : Extracts from Jurkat cells
      Lane 3 : Extracts from Jurkat cells with immunising peptide at 5 µg

      Lysates/proteins at 5 µg per lane.

      Predicted band size : 40 , 42 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 75 kDa,80 kDa,85 kDa. We are unsure as to the identity of these extra bands.
    • ab72603, at 1/500 dilution, staining HepG2 cells in the absence or presence of the immunising peptide.

    Anti-ELAVL2 + ELAVL4 antibody (ab72603)参考文献

    ab72603 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry
    Sample Human Cultured Cells (NPCs derived from iPSC)
    Permeabilization Yes - 0.25% Triton X-100 in PBS
    Specification NPCs derived from iPSC
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative Paraformaldehyde

    Abcam user community

    Verified customer

    提交于 Feb 10 2016

    Thank you for your reply and sorry for the delay in mine.

    I have aligned our exact immunogen sequence for ab136069 with the provided protein sequences. The homology score is listed below:

    Dogfish Elavl4: 92.9% identity in a ...

    Read More

    I can now share some further information with you in regards to the other anti-ELAVL2 + ELAVL4 antibody we have on our catalogue, ab136069.

    The immunogen used to raise this antibody has been taken from within the residues 50-100 of human ELAV2...

    Read More

    Many thanks for contacting us and your interest in our products.

    The exact immunogen used to raise the Anti-ELAVL2 + ELAVL4 antibody (ab72603) is proprietary information. However, I am able to share with you that the immunogen used ...

    Read More