Anti-Elav-type RNA-binding protein ETR3抗体(ab50734)

概述

  • 产品名称Anti-Elav-type RNA-binding protein ETR3抗体
    参阅全部 Elav-type RNA-binding protein ETR3 一抗
  • 描述
    兔多克隆抗体to Elav-type RNA-binding protein ETR3
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Zebrafish
  • 免疫原

    Synthetic peptide: EPYGAVYQIN VLRDRSQNPP QSKGCCFVTF YTRKAALEAQ NALHNIKTLP, corresponding to amino acids 37-86 of Human Elav-type RNA-binding protein ETR3

  • 阳性对照
    • Jurkat cell lysate; Human heart tissue

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab50734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 56 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 功能RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of TNNT2 in embryonic, but not adult, skeletal muscle. Activates TNNT2 exon 5 inclusion by antagonizing the repressive effect of PTB. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Promotes inclusion of exonS 21 and exclusion of exon 5 of the NMDA receptor R1 pre-mRNA. Involved in the apoB RNA editing activity. Increases COX2 mRNA stability and inhibits COX2 mRNA translation in epithelial cells after radiation injury (By similarity). Modulates the cellular apoptosis program by regulating COX2-mediated prostaglandin E2 (PGE2) expression (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK. Binds to the muscle-specific splicing enhancer (MSE) intronic sites flanking the TNNT2 alternative exon 5. Binds preferentially to UG-rich sequences, in particular UG repeat and UGUU motifs. Binds to apoB mRNA, specifically to AU-rich sequences located immediatly upstream of the edited cytidine. Binds AU-rich sequences in the 3'-UTR of COX2 mRNA (By similarity). Binds to an intronic RNA element responsible for the silencing of exon 21 splicing (By similarity). Binds to (CUG)n repeats.
  • 组织特异性Expressed in frontal cortex. Isoform 1 is expressed in brain and lung. Isoform 2 is expressed in heart, brain, placenta, lung, liver, kidney, skeletal muscle and pancreas. Isoform 4 is expressed in heart, lung, skeletal muscle, kidney and pancreas.
  • 序列相似性Belongs to the CELF/BRUNOL family.
    Contains 3 RRM (RNA recognition motif) domains.
  • 发展阶段Isoform 1 is expressed in fetal brain. Isoform 2 is expressed in fetal heart, brain, thymus, lung, liver, skeletal muscle, kidney and spleen. Isoform 4 is expressed in fetal heart, brain, thymus, lung and skeletal muscle.
  • 细胞定位Nucleus. Cytoplasm. Accumulates in the cytoplasm after ionizing radiation (By similarity). Colocalizes with APOBEC1 and A1CF. RNA-binding activity is detected in both nuclear and cytoplasmic compartments.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Bruno-like protein 3 antibody
    • BRUNOL3 antibody
    • Cardiac elav type RNA binding protein antibody
    • CELF-2 antibody
    • celf2 antibody
    • CELF2_HUMAN antibody
    • CUG BP2 antibody
    • CUG triplet repeat RNA-binding protein 2 antibody
    • CUG-BP- and ETR-3-like factor 2 antibody
    • CUG-BP2 antibody
    • CUGBP Elav like family member 2 antibody
    • CUGBP Elav-like family member 2 antibody
    • CUGBP2 antibody
    • dJ323N1.1 antibody
    • elav type RNA binding protein antibody
    • ELAV-type RNA-binding protein 3 antibody
    • ETR 3 antibody
    • ETR-3 antibody
    • ETR3 antibody
    • HGNC:2550 antibody
    • hNAPOR antibody
    • NAPOR 2 antibody
    • NAPOR antibody
    • Neuroblastoma apoptosis-related RNA-binding protein antibody
    • Neuroplastoma apoptosis related RNA binding protein 3 antibody
    • RNA binding protein BRUNOL3 antibody
    • RNA-binding protein BRUNOL-3 antibody
    see all

Anti-Elav-type RNA-binding protein ETR3 antibody 图像

  • Lane 1 : Marker
    Lane 2 : Anti-Elav-type RNA-binding protein ETR3 antibody (ab50734) at 1.25 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 56 kDa
    Observed band size : 61 kDa (why is the actual band size different from the predicted?)
  • ab50734 at a concentration of 4 µg/ml, positively labelling myocardial cells (indicated by arrows) in paraffin-embedded human heart tissue. Magnification 400x

Anti-Elav-type RNA-binding protein ETR3 antibody (ab50734)参考文献

This product has been referenced in:
  • Miyazaki Y  et al. Viral delivery of miR-196a ameliorates the SBMA phenotype via the silencing of CELF2. Nat Med 18:1136-41 (2012). Read more (PubMed: 22660636) »

See 1 Publication for this product

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