概述

  • 产品名称Anti-Elastase抗体
    参阅全部 Elastase 一抗
  • 描述
    兔多克隆抗体to Elastase
  • 经测试应用适用于: IHC-P, IHC-Fr, ELISA, WB, IP, ICCmore details
  • 种属反应性
    与反应: Mouse
    不与反应: Human, Pig
  • 免疫原

    Full length protein: Rat pancreatic elastase.

性能

应用

Our Abpromise guarantee covers the use of ab21593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
IHC-Fr
ELISA
WB
IP
ICC
  • 应用说明ELISA: 1/1000 - 1/2000.
    ICC: 1/50 - 1/100.
    IHC-Fr: 1/1000 (PMID 20003319).
    IP: 1/100 - 1/200.
    WB: 1/200 - 1/500. Predicted molecular weight: 29 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Efficient protease with alanine specificity but only little elastolytic activity.
    • 序列相似性Belongs to the peptidase S1 family. Elastase subfamily.
      Contains 1 peptidase S1 domain.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CEL3A_HUMAN antibody
      • CELA3A antibody
      • Chymotrypsin-like elastase family member 3A antibody
      • ELA1 antibody
      • ELA2A antibody
      • ELA2B antibody
      • ELA3 antibody
      • ELA3A antibody
      • ELA3B antibody
      • Elastase 1 antibody
      • Elastase 2A antibody
      • Elastase 2B antibody
      • Elastase 3A antibody
      • Elastase 3A pancreatic antibody
      • Elastase 3B antibody
      • Elastase 3B pancreatic antibody
      • Elastase IIIA antibody
      • Elastase IIIB antibody
      • Elastase-3A antibody
      • Fecal pancreatic elastase 1 antibody
      • HLE antibody
      • Medullasin antibody
      • Pancreatic elastase IIA antibody
      • PE 1 antibody
      • PE1 antibody
      • Protease E antibody
      see all

    Anti-Elastase antibody 图像

    • ab21593 staining Elastase in whole mouse embryos by Immunohistochemistry (PFA perfusion fixed frozen sections).
      Immunofluorescence was performed on e14.5, Nkx2.2-/- whole embryo frozen 8 µm sections that were fixed overnight with 4% paraformaldehyde. ab21593 was used at 1/1000. DAPI was used at 1/1000 and incubated for 30 minutes. Confocal images were taken on a Zeiss META LSM 510.
    • ab21593 staining Elastase in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/300 in BSA + TBS) for 30 minutes. An undiluted Goat anti-rabbit HRP polyclonal was used as the secondary antibody.

      See Abreview

    Anti-Elastase antibody (ab21593)参考文献

    This product has been referenced in:
    • Hunter KE  et al. Identification and characterization of poorly differentiated invasive carcinomas in a mouse model of pancreatic neuroendocrine tumorigenesis. PLoS One 8:e64472 (2013). IHC-P ; Mouse . Read more (PubMed: 23691228) »
    • Holmstrom SR  et al. LRH-1 and PTF1-L coregulate an exocrine pancreas-specific transcriptional network for digestive function. Genes Dev 25:1674-9 (2011). Read more (PubMed: 21852532) »

    See all 4 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Pancreas)
    Specification Pancreas
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
    Permeabilization No
    Username

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    Verified customer

    提交于 Jun 11 2012

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