EL4全细胞裂解液(ab7183)

概述

  • 产品名称EL4全细胞裂解液
  • 常规说明Cell line: EL4 (T lymphocyte; lymphoma).
    Growth media: DMEM and 10% horse serum.


    Mouse EL4 cell lysate was prepared by homogenization in modified RIPA buffer (50mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • 经测试应用适用于: WBmore details

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: 5% Beta mercaptoethanol, 12.5% Glycerol, 1% Triton-X-100, 0.2% Sodium Deoxycholate, 0.01% Bromophenol blue, 5µg/ml Leupeptin, 5µg/ml Aprotinin, 50mM Tris HCl, 1mM EDTA, 1mM PMSF
  • Concentration information loading...
  • 裂解物说明Mouse EL4 cell lysate was prepared by homogenization in modified RIPA buffer (50mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • 研究领域
  • 背景EL4 cells are derived from ascetic fluid of a C57BL/6N mouse lymphoma and are therefore typed as lymphoblastic. They produce interferon and are resistant to cortisol and dexamethasone. They are used for research in tumorigenicity and virology.

应用

Our Abpromise guarantee covers the use of ab7183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. EL4 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.

EL4 whole cell lysate (ab7183)参考文献

ab7183 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Questions for ab7183.
Please use the link above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"