概述

  • 产品名称Anti-EKLF / KLF1抗体- ChIP Grade
    参阅全部 EKLF / KLF1 一抗
  • 描述
    兔多克隆抗体to EKLF / KLF1 - ChIP Grade
  • 经测试应用适用于: WB, Indirect ELISA, ChIPmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Synthetic peptide corresponding to C terminal residues of KLF1 (Erythroid krueppel like transcription factor) (Mouse)

性能

应用

Our Abpromise guarantee covers the use of ab56011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. Incubate for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
Indirect ELISA Use at an assay dependent dilution.
ChIP Use a concentration of 4 mg/ml. PubMed: 21807894

靶标

  • 功能Transcription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis. Is a dual regulator of fetal-to-adult globin switching. Binds to the CACCC box in the beta-globin gene promoter and acts as a preferential activator of this gene. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes. This dual activity ensures that, in most adults, fetal hemoglobin levels are low. Able to activate CD44 and AQP1 promoters. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation.
  • 组织特异性Expression restricted to adult bone marrow and fetal liver. Not expressed in myeloid nor lymphoid cell lines.
  • 疾病相关Defects in KLF1 are the cause of congenital dyserythropoietic anemia type 4 (CDA4) [MIM:613673]. It is a blood disorder characterized by ineffective erythropoiesis and hemolysis resulting in anemia. Circulating erythroblasts and erythroblasts in the bone marrow show various morphologic abnormalities. Affected individuals with CDA4 also have increased levels of fetal hemoglobin.
  • 序列相似性Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • 翻译后修饰Acetylated; can be acetylated on both Lys-274 and Lys-288. Acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity.
    Sumoylated; sumoylation, promoted by PIAS1, leads to repression of megakaryocyte differentiation. Also promotes the interaction with the CDH4 subunit of the NuRD repression complex.
    Phosphorylated primarily on serine residues in the transactivation domain. Phosphorylation on Thr-23 is critical for the transactivation activity.
  • 细胞定位Nucleus. Colocalizes with SUMO1 in nuclear speckles.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CDAN4 antibody
    • EKLF antibody
    • EKLF PEN antibody
    • Erythroid krueppel like transcription factor (EKLF) (Erythroid transcription factor) antibody
    • Erythroid krueppel-like transcription factor antibody
    • Erythroid Kruppel like factor antibody
    • Erythroid specific transcription factor EKLF antibody
    • HBFQTL6 antibody
    • Human erythroid-specific transcription factor EKLF mRNA complete cds antibody
    • INLU antibody
    • Klf1 antibody
    • KLF1_HUMAN antibody
    • Krueppel-like factor 1 antibody
    • Kruppel like factor 1 (erythroid) antibody
    see all

Anti-EKLF / KLF1 antibody - ChIP Grade (ab56011)参考文献

This product has been referenced in:
  • Porcu S  et al. Klf1 affects DNase II-alpha expression in the central macrophage of a fetal liver erythroblastic island: a non-cell-autonomous role in definitive erythropoiesis. Mol Cell Biol 31:4144-54 (2011). WB, ChIP ; Mouse . Read more (PubMed: 21807894) »

See 1 Publication for this product

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