概述

  • 产品名称Anti-eIF5A2抗体
    参阅全部 eIF5A2 一抗
  • 描述
    小鼠单克隆抗体to eIF5A2
  • 经测试应用适用于: WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: GKKYEDICPS THNMDVPNIK RNDYQLICIQ DGYLSLLTET GEVREDLKLP EGELGKEIEG KYNAGEDVQV SVMCAMSEEY AVAIKPCK, corresponding to amino acids 66-154 of Human eIF5A2

性能

应用

Our Abpromise guarantee covers the use of ab57421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation.
  • 组织特异性Expressed in ovarian and colorectal cancer cell lines (at protein level). Highly expressed in testis. Overexpressed in some cancer cells.
  • 序列相似性Belongs to the eIF-5A family.
  • 翻译后修饰eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).
  • 细胞定位Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 9630038B20 antibody
    • eIF 5A 2 antibody
    • eIF 5A2 antibody
    • eIF-4D antibody
    • eIF-5A-2 antibody
    • eIF-5A2 antibody
    • EIF5A 2 antibody
    • EIF5A2 antibody
    • eIF5AII antibody
    • Eukaryotic initiation factor 5A isoform 2 antibody
    • Eukaryotic translation initiation factor 5A-2 antibody
    • Eukaryotic translation initiation factor 5A2 antibody
    • IF5A2_HUMAN antibody
    see all

Anti-eIF5A2 antibody 图像

  • Western blot against tagged recombinant protein immunogen using ab57421 eIF5A2 antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa
  • Overlay histogram showing HeLa cells stained with ab57421 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57421, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-eIF5A2 antibody (ab57421)参考文献

ab57421 has not yet been referenced specifically in any publications.

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