RabMAb

Anti-eIF5A抗体[EP527Y] (ab32407)

概述

  • 产品名称Anti-eIF5A抗体[EP527Y]
    参阅全部 eIF5A 一抗
  • 描述
    兔单克隆抗体[EP527Y] to eIF5A
  • 经测试应用适用于: WB, IHC-P, Flow Cytmore details
    不适用于: ICC or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human eIF5A aa 50-150 (C terminal). The exact sequence is proprietary.

  • 阳性对照
    • WB: Jurkat cell lysates. IHC-P: human urinary bladder carcinoma.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab32407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明Is unsuitable for ICC or IP.
  • 靶标

    • 功能mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.
    • 组织特异性Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).
    • 序列相似性Belongs to the eIF-5A family.
    • 翻译后修饰eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).
    • 细胞定位Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.
    • Information by UniProt
    • 数据库链接
    • 别名
      • eIF 4D antibody
      • eIF 5A 1 antibody
      • EIF 5A antibody
      • eIF 5A1 antibody
      • eIF-4D antibody
      • eIF-5A antibody
      • eIF-5A-1 antibody
      • eIF-5A1 antibody
      • eIF4D antibody
      • eif5a antibody
      • EIF5A1 antibody
      • eIF5AI antibody
      • Eukaryotic initiation factor 5A antibody
      • Eukaryotic initiation factor 5A isoform 1 antibody
      • Eukaryotic translation initiation factor 5A 1 antibody
      • Eukaryotic translation initiation factor 5A antibody
      • Eukaryotic translation initiation factor 5A-1 antibody
      • IF5A1_HUMAN antibody
      • MGC104255 antibody
      • MGC99547 antibody
      • Rev binding factor antibody
      • Rev-binding factor antibody
      • uORF A antibody
      • uORF antibody
      see all

    Anti-eIF5A antibody [EP527Y] 图像

    • Anti-eIF5A antibody [EP527Y] (ab32407) at 1/1000 dilution + Jurkat cell lysate

      Predicted band size : 18 kDa
      Observed band size : 18 kDa
    • Ab32407, at a dilution of 1/250, staining eIF5A in paraffin embedded human urinary bladder carcinoma tissue sections by Immunohistochemistry.
    • Overlay histogram showing Jurkat cells stained with ab32407 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32407, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    Anti-eIF5A antibody [EP527Y] (ab32407)参考文献

    ab32407 has not yet been referenced specifically in any publications.

    Product Wall

    We have tested ab32407 in IP and in our hands we could not get this to work hence why we have stated on the datasheet that this antibody is unsuitable for use in this application.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"