RabMAb

Anti-eIF5A抗体[EP526Y] (ab32443)

概述

  • 产品名称Anti-eIF5A抗体[EP526Y]
    参阅全部 eIF5A 一抗
  • 描述
    兔单克隆抗体[EP526Y] to eIF5A
  • 经测试应用适用于: IHC-FoFr, WB, IHC-P, Flow Cyt, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Drosophila melanogaster
  • 免疫原

    Synthetic peptide within Human eIF5A aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P63241

  • 阳性对照
    • Jurkat and HeLa cell lysate and Adenocarcinoma of human uterus slides.
  • 常规说明

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab32443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr 1/400. PubMed: 19546244
WB 1/5000 - 1/10000. Predicted molecular weight: 18 kDa.
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/50 - 1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.
ICC/IF 1/500.

靶标

  • 功能mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.
  • 组织特异性Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level).
  • 序列相似性Belongs to the eIF-5A family.
  • 翻译后修饰eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).
  • 细胞定位Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • eIF 4D antibody
    • eIF 5A 1 antibody
    • EIF 5A antibody
    • eIF 5A1 antibody
    • eIF-4D antibody
    • eIF-5A antibody
    • eIF-5A-1 antibody
    • eIF-5A1 antibody
    • eIF4D antibody
    • eif5a antibody
    • EIF5A1 antibody
    • eIF5AI antibody
    • Eukaryotic initiation factor 5A antibody
    • Eukaryotic initiation factor 5A isoform 1 antibody
    • Eukaryotic translation initiation factor 5A 1 antibody
    • Eukaryotic translation initiation factor 5A antibody
    • Eukaryotic translation initiation factor 5A-1 antibody
    • IF5A1_HUMAN antibody
    • MGC104255 antibody
    • MGC99547 antibody
    • Rev binding factor antibody
    • Rev-binding factor antibody
    • uORF A antibody
    • uORF antibody
    see all

Anti-eIF5A antibody [EP526Y] 图像

  • Anti-eIF5A antibody [EP526Y] (ab32443) at 1/10000 dilution + Jurkat cell lysate.

    Predicted band size : 18 kDa
    Observed band size : 18 kDa
  • Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling eIF5A with purified ab32443 at 1/500. Cells were fixed with 4% Paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue). 

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control

  • ab32443 at a 1:250 dilution staining eIF5A in human adenocarcinoma of uterus using Immunohistochemistry, Paraffin Embedded Tissue.
  • Overlay histogram showing Jurkat cells stained with ab32443 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32443, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (0.5µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-eIF5A antibody [EP526Y] (ab32443)参考文献

This product has been referenced in:
  • Fujimura K  et al. A hypusine-eIF5A-PEAK1 switch regulates the pathogenesis of pancreatic cancer. Cancer Res 74:6671-81 (2014). Read more (PubMed: 25261239) »
  • Patel PH  et al. The Drosophila deoxyhypusine hydroxylase homologue nero and its target eIF5A are required for cell growth and the regulation of autophagy. J Cell Biol 185:1181-94 (2009). WB, IHC-FoFr . Read more (PubMed: 19546244) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.

The immunogen shares 74% identity with human EIF5A2, so it is not predicted to cross-react.

Please do not hesitate to contact us if you need any more advice or information.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HCT116 colon cancer cell line)
Loading amount 30 µg
Specification HCT116 colon cancer cell line
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% in TBST
Username

Abcam user community

Verified customer

提交于 Nov 07 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"