Anti-EIF5抗体(ab85913)
Key features and details
- Rabbit polyclonal to EIF5
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
-
产品名称
Anti-EIF5抗体
参阅全部 EIF5 一抗 -
描述
兔多克隆抗体to EIF5 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Turkey, Xenopus laevis, Chimpanzee, Rhesus monkey, Tilapia, Orangutan, Xenopus tropicalis, Medaka fish -
免疫原
Synthetic peptide, corresponding to a region between residues 100 and 150 of Human EIF5 (NP_892116.2)
-
阳性对照
- HeLa, 293T and NIH3T3 whole cell lysates.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
ab85913 was affinity purified using an epitope specific to EIF5 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab85913于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/2000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
|
说明 |
---|
WB
1/2000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). |
靶标
-
功能
Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]). -
序列相似性
Belongs to the eIF-2-beta/eIF-5 family.
Contains 1 W2 domain. - Information by UniProt
-
数据库链接
- Entrez Gene: 423479 Chicken
- Entrez Gene: 504752 Cow
- Entrez Gene: 1983 Human
- Entrez Gene: 100047658 Mouse
- Entrez Gene: 217869 Mouse
- Entrez Gene: 56783 Rat
- Entrez Gene: 380003 Xenopus laevis
- Entrez Gene: 493571 Xenopus tropicalis
see all -
别名
- 2810011H21Rik antibody
- D12Ertd549e antibody
- EIF 5 antibody
see all
图片
-
All lanes : Anti-EIF5 antibody (ab85913) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 49 kDa
Observed band size: 49 kDa
Detection: Chemiluminescence
数据表及文件
-
SDS download
-
Datasheet download
文献 (4)
ab85913 被引用在 4 文献中.
- Loughran G et al. Translational autoregulation of BZW1 and BZW2 expression by modulating the stringency of start codon selection. PLoS One 13:e0192648 (2018). PubMed: 29470543
- Tzani I et al. Systematic analysis of the PTEN 5' leader identifies a major AUU initiated proteoform. Open Biol 6:N/A (2016). PubMed: 27249819
- Chen H et al. Levels of the EMT-related protein Snail/Slug are not correlated with p53/p63 in cutaneous squamous cell carcinoma. J Cutan Pathol 40:651-6 (2013). PubMed: 23521708
- Loughran G et al. Stringency of start codon selection modulates autoregulation of translation initiation factor eIF5. Nucleic Acids Res : (2011). WB ; Human . PubMed: 22156057