概述

  • 产品名称Anti-EIF5抗体
    参阅全部 EIF5 一抗
  • 描述
    小鼠单克隆抗体to EIF5
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein, Human EIF5 (432 aa) with 26 kDa enzyme tag (AAH32866).

  • 阳性对照
    • Recombinant full length human EIF5.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab76633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged EIF5 is approximately 10ng/ml if used as a capture antibody.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 49 kDa).

靶标

  • 功能Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).
  • 序列相似性Belongs to the eIF-2-beta/eIF-5 family.
    Contains 1 W2 domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2810011H21Rik antibody
    • D12Ertd549e antibody
    • EIF 5 antibody
    • EIF 5A antibody
    • eIF-5 antibody
    • Eif5 antibody
    • Eukaryotic initiation factor 5 antibody
    • Eukaryotic translation initiation factor 5 antibody
    • IF5_HUMAN antibody
    • MGC36374 antibody
    • MGC36509 antibody
    see all

Anti-EIF5 antibody 图像

  • Anti-EIF5 antibody (ab76633) at 5 µg/ml + Recombinant full length human EIF5 with 26 kDa tag, at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 49 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)

Anti-EIF5 antibody (ab76633)参考文献

ab76633 has not yet been referenced specifically in any publications.

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