概述

  • 产品名称Anti-eIF4H抗体
    参阅全部 eIF4H 一抗
  • 描述
    兔多克隆抗体to eIF4H
  • 经测试应用适用于: IHC-P, WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    A region between residue 198 and 248 of human eIF4H using the numbering given in entry NP_071496.1

  • 阳性对照
    • HeLa cells lysate 293T cells lysate
  • 常规说明Shelf Life: 1 year from date of receipt

性能

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应用

Our Abpromise guarantee covers the use of ab86179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 27 kDa.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Stimulates the RNA helicase activity of EIF4A in the translation initiation complex. Binds weakly mRNA.
    • 组织特异性The short isoform is the predominant isoform and is expressed alone in liver and skeletal muscle. Both isoforms are expressed in fibroblast, spleen, testis and bone marrow. Levels are high in lung and pancreas and low in heart, frontal cortex and kidney.
    • 疾病相关Note=EIF4H is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of EIF4H may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
    • 序列相似性Contains 1 RRM (RNA recognition motif) domain.
    • 细胞定位Cytoplasm > perinuclear region.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AU018978 antibody
      • D5Ertd355e antibody
      • E430026L18Rik antibody
      • Ef4h antibody
      • eIF 4H antibody
      • eIF-4H antibody
      • EIF4H antibody
      • Eukaryotic translation initiation factor 4H antibody
      • IF4H_HUMAN antibody
      • KIAA0038 antibody
      • WBSCR1 antibody
      • Williams Beuren syndrome chromosomal region 1 protein homolog antibody
      • Williams Beuren syndrome chromosome region 1 antibody
      • Williams-Beuren syndrome chromosomal region 1 protein antibody
      • Williams-Beuren syndrome chromosome region 1 antibody
      • WSCR1 antibody
      see all

    Anti-eIF4H antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling eIF4H with ab86179 at 1/200 (1µg/ml). Detection: DAB.
    • All lanes : Anti-eIF4H antibody (ab86179) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 27 kDa


      Exposure time : 3 minutes

    Anti-eIF4H antibody (ab86179)参考文献

    ab86179 has not yet been referenced specifically in any publications.

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