Anti-eIF4H抗体(ab77455)

概述

  • 产品名称Anti-eIF4H抗体
    参阅全部 eIF4H 一抗
  • 描述
    小鼠单克隆抗体to eIF4H
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Chimpanzee, Rhesus monkey
  • 免疫原

    Recombinant fragment: MADFDTYDDR AYSSFGGGRG SRGSAGGHGS RSQKELPTEP PYTAYVGNLP FNTVQGDIDA IFKDLSIRSV RLVRDKDTDK FKGFCYVEFD EVDSLKEALT, corresponding to amino acids 1-100 of Human eIF4H (NP_114381) with a 26 kDa tag.

  • 阳性对照
    • eIF4H transfected 293T cell lysate; Recombinant tagged human eIF4H fragment.

性能

应用

Our Abpromise guarantee covers the use of ab77455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 27 kDa.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged eIF4H is approximately 3ng/ml when used as a capture antibody.

靶标

  • 功能Stimulates the RNA helicase activity of EIF4A in the translation initiation complex. Binds weakly mRNA.
  • 组织特异性The short isoform is the predominant isoform and is expressed alone in liver and skeletal muscle. Both isoforms are expressed in fibroblast, spleen, testis and bone marrow. Levels are high in lung and pancreas and low in heart, frontal cortex and kidney.
  • 疾病相关Note=EIF4H is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of EIF4H may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
  • 序列相似性Contains 1 RRM (RNA recognition motif) domain.
  • 细胞定位Cytoplasm > perinuclear region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AU018978 antibody
    • D5Ertd355e antibody
    • E430026L18Rik antibody
    • Ef4h antibody
    • eIF 4H antibody
    • eIF-4H antibody
    • EIF4H antibody
    • Eukaryotic translation initiation factor 4H antibody
    • IF4H_HUMAN antibody
    • KIAA0038 antibody
    • WBSCR1 antibody
    • Williams Beuren syndrome chromosomal region 1 protein homolog antibody
    • Williams Beuren syndrome chromosome region 1 antibody
    • Williams-Beuren syndrome chromosomal region 1 protein antibody
    • Williams-Beuren syndrome chromosome region 1 antibody
    • WSCR1 antibody
    see all

Anti-eIF4H antibody 图像

  • Anti-eIF4H antibody (ab77455) at 1 µg/ml + Recombinant tagged human eIF4H fragment at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 27 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-eIF4H antibody (ab77455) at 1 µg/ml

    Lane 1 : eIF4H transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 27 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

Anti-eIF4H antibody (ab77455)参考文献

ab77455 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (hela)
Loading amount 35 µg
Specification hela
Gel Running Conditions Reduced Denaturing (17.5)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Mar 02 2012

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