概述

  • 产品名称
    Anti-eIF4GII抗体
    参阅全部 eIF4GII 一抗
  • 描述
    兔多克隆抗体to eIF4GII
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 1152-1201 (

    MRGGSSKDLLDNQSQEEQRREMLETVKQLTGGVDVERNSTEAERNKTRES

    ) of human EIF4G3 (NP_003751).

  • 阳性对照
    • HeLa cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab83330 is purified by peptide affinity chromatography method.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab83330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 177 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titer in peptide based assay is 1/1562500.

靶标

  • 功能
    Probable component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Thought to be a functional homolog of EIF4G1.
  • 序列相似性
    Belongs to the eukaryotic initiation factor 4G family.
    Contains 5 HEAT repeats.
    Contains 1 MI domain.
    Contains 1 MIF4G domain.
    Contains 1 W2 domain.
  • 翻译后修饰
    Following infection by certain enteroviruses, rhinoviruses and aphthoviruses, EIF4G1 is cleaved by the viral protease 2A, or the leader protease in the case of aphthoviruses. This shuts down the capped cellular mRNA transcription.
  • Information by UniProt
  • 数据库链接
  • 别名
    • eIF-4-gamma 3 antibody
    • eIF-4-gamma II antibody
    • eIF-4G 3 antibody
    • eIF4G 3 antibody
    • EIF4G3 antibody
    • eIF4GII antibody
    • Eukaryotic translation initiation factor 4 gamma 3 antibody
    • eukaryotic translation initiation factor 4 gamma, 3 antibody
    • eukaryotic translation initiation factor-4 gamma, 3 antibody
    • IF4G3_HUMAN antibody
    see all

Anti-eIF4GII antibody 图像

  • Anti-eIF4GII antibody (ab83330) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 177 kDa
    Observed band size : 160 kDa (why is the actual band size different from the predicted?)
    Additional bands at : <40 kDa. We are unsure as to the identity of these extra bands.

实验方案

Anti-eIF4GII antibody (ab83330)参考文献

ab83330 has not yet been referenced specifically in any publications.

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