概述

  • 产品名称Anti-eIF4G1抗体
    参阅全部 eIF4G1 一抗
  • 描述
    兔多克隆抗体to eIF4G1
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human eIF4G1.

    (Peptide available as ab47648.)

  • 阳性对照
    • This antibody gave a positive signal in HEK293 Human embryonic kidney cell line Whole Cell Lysate.

性能

应用

Our Abpromise guarantee covers the use of ab47649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 220 kDa (predicted molecular weight: 220 kDa).

靶标

  • 功能Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome.
  • 疾病相关Defects in EIF4G1 are the cause of Parkinson disease type 18 (PARK18) [MIM:614251]. An autosomal dominant, late-onset form of Parkinson disease. Parkinson disease is a complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain.
  • 序列相似性Belongs to the eIF4G family.
    Contains 1 MI domain.
    Contains 1 MIF4G domain.
    Contains 1 W2 domain.
  • 翻译后修饰Phosphorylated at multiple sites in vivo. Phosphorylation at Ser-1185 by PRKCA induces binding to MKNK1.
    Following infection by certain enteroviruses, rhinoviruses and aphthoviruses, EIF4G1 is cleaved by the viral protease 2A, or the leader protease in the case of aphthoviruses. This shuts down the capped cellular mRNA transcription.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp686A1451 antibody
    • eIF 4 gamma 1 antibody
    • eIF 4G 1 antibody
    • eIF 4G1 antibody
    • eIF-4-gamma 1 antibody
    • eIF-4G 1 antibody
    • eIF-4G1 antibody
    • EIF4 gamma antibody
    • EIF4F antibody
    • EIF4G antibody
    • EIF4G1 antibody
    • EIF4GI antibody
    • Eukaryotic translation initiation factor 4 gamma 1 antibody
    • IF4G1_HUMAN antibody
    • p220 antibody
    see all

Anti-eIF4G1 antibody 图像

  • Anti-eIF4G1 antibody (ab47649) at 1 µg/ml + HEK293 Human embryonic kidney cell line Whole Cell Lysate at 10 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 220 kDa
    Observed band size : 220 kDa
    Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab47649 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab47649, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

Anti-eIF4G1 antibody (ab47649)参考文献

This product has been referenced in:
  • Sinvani H  et al. Translational tolerance of mitochondrial genes to metabolic energy stress involves TISU and eIF1-eIF4GI cooperation in start codon selection. Cell Metab 21:479-92 (2015). WB ; Human . Read more (PubMed: 25738462) »
  • Kaehler C  et al. 5-Fluorouracil affects assembly of stress granules based on RNA incorporation. Nucleic Acids Res 42:6436-47 (2014). ICC/IF ; Human . Read more (PubMed: 24728989) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (293T cells)
Gel Running Conditions Reduced Denaturing (8%)
Loading amount 20 µg
Treatment 50 nM Dharmacon siGENOME SMART pool eIF4GI siRNA for 72h
Specification 293T cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jul 16 2015

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