概述

  • 产品名称Anti-eIF4ENIF1抗体
    参阅全部 eIF4ENIF1 一抗
  • 描述
    兔多克隆抗体to eIF4ENIF1
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis , Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide, corresponding to a region between residue 925 and the C-terminus (residue 985) of Human eIF4ENIF1 (NP_062817.1).

  • 阳性对照
    • HeLa whole cell lysate, 293T whole cell lysate and mouse NIH3T3 whole cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab95030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 108 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Nucleoplasmic shuttling protein. Mediates the nuclear import of EIF4E by a piggy-back mechanism.
  • 组织特异性Widely expressed.
  • 细胞定位Cytoplasm. Nucleus. Predominantly cytoplasmic. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2610509L04Rik antibody
    • 4E T antibody
    • 4E-T antibody
    • 4ET antibody
    • 4ET_HUMAN antibody
    • A930019J01Rik antibody
    • AA410001 antibody
    • AU021239 antibody
    • Clast 4 antibody
    • Clast4 antibody
    • D11Ertd166e antibody
    • eIF4E transporter antibody
    • Eif4enif1 antibody
    • Eukaryotic translation initiation factor 4E nuclear import factor 1 antibody
    • Eukaryotic translation initiation factor 4E transporter antibody
    • FLJ21601 antibody
    • FLJ26551 antibody
    • OTTMUSP00000000698 antibody
    • OTTMUSP00000005193 antibody
    • OTTMUSP00000005194 antibody
    • OTTMUSP00000036075 antibody
    see all

Anti-eIF4ENIF1 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling eIF4ENIF1 with ab95030 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-eIF4ENIF1 antibody (ab95030) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 108 kDa


    Exposure time : 3 minutes
  • Detection of eIF4ENIF1 in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab95030 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot.

    Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-eIF4ENIF1 antibody (ab95030)参考文献

ab95030 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab95030.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"