Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus
Synthetic peptide, corresponding to a region between residue 925 and the C-terminus (residue 985) of Human eIF4ENIF1 (NP_062817.1).
HeLa whole cell lysate, 293T whole cell lysate and mouse NIH3T3 whole cell lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 108 kDa.
Use at 2-5 µg/mg of lysate.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Nucleoplasmic shuttling protein. Mediates the nuclear import of EIF4E by a piggy-back mechanism.
Cytoplasm. Nucleus. Predominantly cytoplasmic. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner.
Information by UniProt
4E T antibody
Anti-eIF4ENIF1 antibody 图像
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4ENIF1 antibody (ab95030)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling eIF4ENIF1 with ab95030 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - eIF4ENIF1 antibody (ab95030)
All lanes : Anti-eIF4ENIF1 antibody (ab95030) at 1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg Developed using the ECL technique Predicted band size : 108 kDa Exposure time : 3 minutes
Immunoprecipitation - eIF4ENIF1 antibody (ab95030)
Detection of eIF4ENIF1 in immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab95030 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 30 seconds.
Anti-eIF4ENIF1 antibody (ab95030)参考文献
has not yet been referenced specifically in any publications.
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