概述

  • 产品名称Anti-eIF4ENIF1抗体
    参阅全部 eIF4ENIF1 一抗
  • 描述
    山羊多克隆抗体to eIF4ENIF1
  • 经测试应用适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide:

    AKVISVDELEYRQ

    , corresponding to C terminal amino acids 972-985 of Human eIF4ENIF1.

  • 阳性对照
    • HEK293 Lysate.
  • 常规说明GenBank Accession Number – NP_062817.

性能

应用

Our Abpromise guarantee covers the use of ab6034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 22090346
WB Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 140-150 kDa. The predicted molecular weight is 108 kDa according to NP_062817, however our observation agrees with that of Dostie et al, PMID: 10856257.

靶标

  • 功能Nucleoplasmic shuttling protein. Mediates the nuclear import of EIF4E by a piggy-back mechanism.
  • 组织特异性Widely expressed.
  • 细胞定位Cytoplasm. Nucleus. Predominantly cytoplasmic. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2610509L04Rik antibody
    • 4E T antibody
    • 4E-T antibody
    • 4ET antibody
    • 4ET_HUMAN antibody
    • A930019J01Rik antibody
    • AA410001 antibody
    • AU021239 antibody
    • Clast 4 antibody
    • Clast4 antibody
    • D11Ertd166e antibody
    • eIF4E transporter antibody
    • Eif4enif1 antibody
    • Eukaryotic translation initiation factor 4E nuclear import factor 1 antibody
    • Eukaryotic translation initiation factor 4E transporter antibody
    • FLJ21601 antibody
    • FLJ26551 antibody
    • OTTMUSP00000000698 antibody
    • OTTMUSP00000005193 antibody
    • OTTMUSP00000005194 antibody
    • OTTMUSP00000036075 antibody
    see all

Anti-eIF4ENIF1 antibody 图像

  • ab6034 at 0.25µg/ml staining approximately 140-150kDa band of EIF4ENIF1 in HEK293 Lysate by Western blot (ECL).
  • ab6034 at 5ug/ml staining eIF4ENIF1 in human pancreas tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.

Anti-eIF4ENIF1 antibody (ab6034)参考文献

This product has been referenced in:
  • Waghray S  et al. Xenopus CAF1 requires NOT1-mediated interaction with 4E-T to repress translation in vivo. RNA 21:1335-45 (2015). Read more (PubMed: 26015597) »
  • Marnef A  et al. RNA-related nuclear functions of human Pat1b, the P-body mRNA decay factor. Mol Biol Cell 23:213-24 (2012). ICC/IF ; Human . Read more (PubMed: 22090346) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 15 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Jul 19 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"