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Our Abpromise guarantee covers the use of ab6034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 22090346|
|WB||Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 140-150 kDa. The predicted molecular weight is 108 kDa according to NP_062817, however our observation agrees with that of Dostie et al, PMID: 10856257.|
ab6034 at 5ug/ml staining eIF4ENIF1 in human pancreas tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.
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