概述

  • 产品名称Anti-eIF4ENIF1抗体
    参阅全部 eIF4ENIF1 一抗
  • 描述
    兔多克隆抗体to eIF4ENIF1
  • 经测试应用适用于: WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    MDRRSMGETESGDAFLDLK-C

    conjugated to KLH, corresponding to N terminal amino acids 1-19 of Human eIF4ENIF1.

  • 阳性对照
    • HeLa cell lysates HEK-293T cells
  • 常规说明If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

性能

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应用

Our Abpromise guarantee covers the use of ab55881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
ICC/IF
  • 应用说明IP: Use 5-10 µl, using HeLa cell lysate.
    ICC/IF: Use at a concentration of 5 - 10 µg/ml, using paraformaldehyde-fixed HEK-293T cells.
    WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 108 kDa using HeLa cell lysate. Staining of eIF4ENIF1 in immunoblotting is specifically inhibited by the immunizing peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Nucleoplasmic shuttling protein. Mediates the nuclear import of EIF4E by a piggy-back mechanism.
    • 组织特异性Widely expressed.
    • 细胞定位Cytoplasm. Nucleus. Predominantly cytoplasmic. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 2610509L04Rik antibody
      • 4E T antibody
      • 4E-T antibody
      • 4ET antibody
      • 4ET_HUMAN antibody
      • A930019J01Rik antibody
      • AA410001 antibody
      • AU021239 antibody
      • Clast 4 antibody
      • Clast4 antibody
      • D11Ertd166e antibody
      • eIF4E transporter antibody
      • Eif4enif1 antibody
      • Eukaryotic translation initiation factor 4E nuclear import factor 1 antibody
      • Eukaryotic translation initiation factor 4E transporter antibody
      • FLJ21601 antibody
      • FLJ26551 antibody
      • OTTMUSP00000000698 antibody
      • OTTMUSP00000005193 antibody
      • OTTMUSP00000005194 antibody
      • OTTMUSP00000036075 antibody
      see all

    Anti-eIF4ENIF1 antibody 图像

    • Lanes 1, 3 & 4 : Anti-eIF4ENIF1 antibody (ab55881) at 1 µg/ml
      Lane 2 : Anti-eIF4ENIF1 antibody (ab55881) at 2 µg/ml

      Lane 1 : Extracts of HeLa cells
      Lane 2 : Extracts of HeLa cells
      Lane 3 : Extracts of HeLa cells with eIF4ENIF1 immunizing peptide at 10 µg/ml
      Lane 4 : Extracts of HeLa cells with Non-relevant peptide at 10 µg/ml

      Secondary
      HRP-conjugated Goat anti-Rabbit IgG
      Developed using the ECL technique

      Predicted band size : 108 kDa
    • IF localization of eIF4ENIF1 in HEK-293T cells stained with ab55881 at 5 mg/ml (A), and DAPI (B). The antibody was detected with a FITC conjugated Goat anti-Rabbit IgG.
    • All lanes : Anti-eIF4ENIF1 antibody (ab55881) at 1/1000 dilution

      Lane 1 : Whole cell lysate prepared from HeLa cells
      Lane 2 : siRNA knockdown

      Lysates/proteins at 10 µg per lane.

      Secondary
      IRDye 800CW conjugated goat anti-rabbit polyclonal at 1/15000 dilution

      Predicted band size : 108 kDa

      Image courtesy of an anonymous Abreview.

      See Abreview

    Anti-eIF4ENIF1 antibody (ab55881)参考文献

    This product has been referenced in:
    • Cargnello M  et al. Phosphorylation of the eukaryotic translation initiation factor 4E-transporter (4E-T) by c-Jun N-terminal kinase promotes stress-dependent P-body assembly. Mol Cell Biol 32:4572-84 (2012). Read more (PubMed: 22966201) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Sample Mouse Cell lysate - whole cell (n2a mouse neuroblastoma)
    Loading amount 30 µg
    Specification n2a mouse neuroblastoma
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Jul 18 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa)
    Loading amount 10 µg
    Specification HeLa
    Gel Running Conditions Reduced Denaturing (7.5)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Jun 09 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"