概述

  • 产品名称Anti-eIF4ENIF1抗体
    参阅全部 eIF4ENIF1 一抗
  • 描述
    兔多克隆抗体to eIF4ENIF1
  • 特异性ab55880 specifically recognizes human eIF4ENIF1
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide:

    KPQVDRTRDGFRNRQ

    conjugated to KLH, corresponding to amino acids 650-664 of Human eIF4ENIF1

  • 阳性对照
    • HeLa cytoplasmic or nuclear lysate
  • 常规说明If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

性能

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应用

Our Abpromise guarantee covers the use of ab55880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use 5µl for 1010 cells.
    WB: Use at a concentration of 2 - 4 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 108 kDa).
    Staining of eIF4ENIF1 in immunoblotting is specifically inhibited by the immunizing peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Nucleoplasmic shuttling protein. Mediates the nuclear import of EIF4E by a piggy-back mechanism.
    • 组织特异性Widely expressed.
    • 细胞定位Cytoplasm. Nucleus. Predominantly cytoplasmic. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 2610509L04Rik antibody
      • 4E T antibody
      • 4E-T antibody
      • 4ET antibody
      • 4ET_HUMAN antibody
      • A930019J01Rik antibody
      • AA410001 antibody
      • AU021239 antibody
      • Clast 4 antibody
      • Clast4 antibody
      • D11Ertd166e antibody
      • eIF4E transporter antibody
      • Eif4enif1 antibody
      • Eukaryotic translation initiation factor 4E nuclear import factor 1 antibody
      • Eukaryotic translation initiation factor 4E transporter antibody
      • FLJ21601 antibody
      • FLJ26551 antibody
      • OTTMUSP00000000698 antibody
      • OTTMUSP00000005193 antibody
      • OTTMUSP00000005194 antibody
      • OTTMUSP00000036075 antibody
      see all

    Anti-eIF4ENIF1 antibody 图像

    • All lanes : Anti-eIF4ENIF1 antibody (ab55880) at 2 µg/ml

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : Jurkat cell lysate
      Lane 6 : K562 cell lysate

      Secondary
      HRP conjugated Goat-anti Rabbit IgG
      Developed using the ECL technique

      Predicted band size : 108 kDa
      Observed band size : 140 kDa (why is the actual band size different from the predicted?)

    Anti-eIF4ENIF1 antibody (ab55880)参考文献

    ab55880 has not yet been referenced specifically in any publications.

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