产品名称 Anti-EIF4EL3抗体 参阅全部 EIF4EL3 一抗
WB, ELISA, IHC-P more details
KLH conjugated synthetic peptide selected from the N-terminal region of human EIF4EL3.
293 cell lysate or human breast carcinoma tissue.
存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液 Preservative: 0.09% Sodium Azide
Concentration information loading...
纯度 Protein A purified
纯化说明 This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).
1/50 - 1/100.
功能 Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
序列相似性 Belongs to the eukaryotic initiation factor 4E family.
翻译后修饰 Ubiquitinated by ARIH1, leading to its degradation by the proteasome.
Information by UniProt
4E LP antibody
Anti-EIF4EL3 antibody 图像
Western blot - EIF4EL3 antibody (ab63062)
All lanes : Anti-EIF4EL3 antibody (ab63062) at 1/100 dilution Lane 1 : 293 cell lysate, nontransfected Lane 2 : 293 cell lysate, transiently transfected with the EIF4EL3 gene Lysates/proteins at 2 µg per lane. Predicted band size : 28 kDa Observed band size : 30 kDa ( why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EIF4EL3 antibody (ab63062)
Ab63062 (1/50-1/100) staining human EIF4EL3 in human breast carcinoma tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.
Ab63062 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Anti-EIF4EL3 antibody (ab63062)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"