概述

  • 产品名称Anti-EIF4EL3抗体
    参阅全部 EIF4EL3 一抗
  • 描述
    小鼠单克隆抗体to EIF4EL3
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-246 of Human EIF4EL3

性能

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应用

Our Abpromise guarantee covers the use of ab56637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 28 kDa.

靶标

  • 功能Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
  • 序列相似性Belongs to the eukaryotic initiation factor 4E family.
  • 翻译后修饰Ubiquitinated by ARIH1, leading to its degradation by the proteasome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 4E LP antibody
    • 4EHP antibody
    • 4ELP antibody
    • EIF 4E type 2 antibody
    • eIF-4E type 2 antibody
    • EIF4 E2 antibody
    • EIF4E 2 antibody
    • eIF4E family, member 2 antibody
    • EIF4E HOMOLOGOUS PROTEIN antibody
    • EIF4E like cap binding protein antibody
    • EIF4E like protein 4E LP antibody
    • EIF4E like protein 4ELP antibody
    • eIF4E type 2 antibody
    • EIF4E type 3 antibody
    • eIF4E-like 3 antibody
    • eIF4E-like protein 4E-LP antibody
    • EIF4E2 antibody
    • Eukaryotic translation initiation factor 4E family member 2 antibody
    • Eukaryotic translation initiation factor 4E homologous protein antibody
    • Eukaryotic translation initiation factor 4E like 3 antibody
    • Eukaryotic translation initiation factor 4E member 2 antibody
    • Eukaryotic translation initiation factor 4E type 2 antibody
    • Eukaryotic translation initiation factor 4E-like 3 antibody
    • IF 4e antibody
    • IF4E antibody
    • IF4E2_HUMAN antibody
    • mRNA cap binding protein 4EHP antibody
    • mRNA cap binding protein type 3 antibody
    • mRNA cap-binding protein 4EHP antibody
    • mRNA cap-binding protein type 3 antibody
    see all

Anti-EIF4EL3 antibody 图像



  • Predicted band size : 28 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: EIF4EL3 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: HepG2 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab56637 observed at 28 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab56637 was shown to recognize EIF4EL3 when EIF4EL3 knockout samples were used, along with additional cross-reactive bands. Wild-type and EIF4EL3 knockout samples were subjected to SDS-PAGE. ab56637 and ab181602 (loading control to GAPDH) were diluted 5 µg/mL and 1/10 000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.



  • Predicted band size : 28 kDa
    EIF4EL3 antibody (ab56637) at 1ug/lane + HeLa cell lysate at 25ug/lane.

Anti-EIF4EL3 antibody (ab56637)参考文献

ab56637 has not yet been referenced specifically in any publications.

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