概述

  • 产品名称Anti-EIF4EBP3抗体
    参阅全部 EIF4EBP3 一抗
  • 描述
    小鼠单克隆抗体to EIF4EBP3
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Recombinant fragment: MSTSTSCPIP GGRDQLPDCY STTPGGTLYA TTPGGTRIIY DRKFLLECKN SPIARTPPCC LPQIPGVTTP PTAPLSKLEE LKEQETEEEI PDDAQFEMDI , corresponding to amino acids 1-101 of Human EIF4EBP3 (NP_003723) with tag.

  • 阳性对照
    • EIF4EBP3 transfected 293T cell lysate. Recombinant tagged EIF4EBP3 protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab76476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged EIF4EBP3 is approximately 100ng/ml if used as a capture antibody.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 11 kDa.

靶标

Anti-EIF4EBP3 antibody 图像

  • Anti-EIF4EBP3 antibody (ab76476) at 1 µg/ml + Recombinant tagged EIF4EBP3 protein at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 11 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-EIF4EBP3 antibody (ab76476) at 1 µg/ml

    Lane 1 : EIF4EBP3 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 11 kDa
    Observed band size : 16 kDa (why is the actual band size different from the predicted?)

Anti-EIF4EBP3 antibody (ab76476)参考文献

ab76476 has not yet been referenced specifically in any publications.

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