RabMAb

Anti-eIF4EBP1 (phospho T70)抗体[EPR654(2)A] (ab75831)

概述

  • 产品名称Anti-eIF4EBP1 (phospho T70)抗体[EPR654(2)A]
    参阅全部 eIF4EBP1 一抗
  • 描述
    兔单克隆抗体[EPR654(2)A] to eIF4EBP1 (phospho T70)
  • 特异性ab75831 detects eIF4EBP1 phosphorylated at threonine 70.
  • 经测试应用适用于: IHC-P, WBmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding threonine 70 of human eIF4EBP1.

  • 阳性对照
    • 293T cell lysates treated with FBS.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab75831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 13 kDa).
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
    • 序列相似性Belongs to the eIF4E-binding protein family.
    • 翻译后修饰Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 4E-BP1 antibody
      • 4EBP1 antibody
      • 4EBP1_HUMAN antibody
      • BP 1 antibody
      • eIF4E binding protein 1 antibody
      • eIF4E-binding protein 1 antibody
      • Eif4ebp1 antibody
      • Eukaryotic translation initiation factor 4E-binding protein 1 antibody
      • PHAS-I antibody
      • PHASI antibody
      • Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
      see all

    Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A] 图像

    • All lanes : Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A] (ab75831) at 1/500 dilution

      Lane 1 : 293T cell lysates un-treated
      Lane 2 : 293T cell lysates treated with FBS

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 13 kDa
      Observed band size : 17 kDa (why is the actual band size different from the predicted?)

    Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A] (ab75831)参考文献

    ab75831 has not yet been referenced specifically in any publications.

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