Anti-eIF4EBP1 (phospho T46)抗体(ab27792)

概述

  • 产品名称Anti-eIF4EBP1 (phospho T46)抗体
    参阅全部 eIF4EBP1 一抗
  • 描述
    兔多克隆抗体to eIF4EBP1 (phospho T46)
  • 特异性Ab27792 recognises eIF4EBP1 that is phosphorylated at threonine 46.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic phosphopeptide derived from the region of human eIF4EBP1 that contains threonine 46. The sequence is conserved in human, mouse and rat.

  • 阳性对照
    • Human embryonic kidney (HEK293) cells stimulated with EGF or 15% serum, or NIH3T3 cells stimulated with PDGF.

性能

应用

Our Abpromise guarantee covers the use of ab27792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 13 kDa.

靶标

  • 功能Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
  • 序列相似性Belongs to the eIF4E-binding protein family.
  • 翻译后修饰Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 4E-BP1 antibody
    • 4EBP1 antibody
    • 4EBP1_HUMAN antibody
    • BP 1 antibody
    • eIF4E binding protein 1 antibody
    • eIF4E-binding protein 1 antibody
    • Eif4ebp1 antibody
    • Eukaryotic translation initiation factor 4E-binding protein 1 antibody
    • PHAS-I antibody
    • PHASI antibody
    • Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
    see all

Anti-eIF4EBP1 (phospho T46) antibody 图像

  • All lanes : Anti-eIF4EBP1 (phospho T46) antibody (ab27792) at 1/1000 dilution (diluted in 3% BSA TBST buffer, incubated for two hours at room temperature.)

    Lane 1 : PDGF treated NIH3T3 cell lysate
    Lane 2 : PI3K inhibitor LY2904002 treated NIH3T3 cell lysate
    Lane 3 : PI3K inhibitor LY2904002 and PDGF treated NIH3T3 cell lysate

    Secondary
    goat F(ab)2 anti rabbit IgG HRP conjugate

    Predicted band size : 13 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)

Anti-eIF4EBP1 (phospho T46) antibody (ab27792)参考文献

ab27792 has not yet been referenced specifically in any publications.

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