Anti-eIF4EBP1 (phospho T45)抗体(ab47367)

概述

  • 产品名称Anti-eIF4EBP1 (phospho T45)抗体
    参阅全部 eIF4EBP1 一抗
  • 描述
    兔多克隆抗体to eIF4EBP1 (phospho T45)
  • 特异性ab47367 detects endogenous levels of 4EBP1 only when phosphorylated at threonine 45. This antibody may cross-react with 4EBP2 and 4EBP3 when phosphorylated at equivalent sites.
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    The antiserum was produced against synthesized phosphopeptide derived from human 4EBP1 around the phosphorylation site of threonine 45 (S-T-TP-P-G).

  • 阳性对照
    • MDA435 cells, breast carcinoma tissue

性能

应用

Our Abpromise guarantee covers the use of ab47367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 13 kDa.
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

靶标

  • 功能Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
  • 序列相似性Belongs to the eIF4E-binding protein family.
  • 翻译后修饰Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 4E-BP1 antibody
    • 4EBP1 antibody
    • 4EBP1_HUMAN antibody
    • BP 1 antibody
    • eIF4E binding protein 1 antibody
    • eIF4E-binding protein 1 antibody
    • Eif4ebp1 antibody
    • Eukaryotic translation initiation factor 4E-binding protein 1 antibody
    • PHAS-I antibody
    • PHASI antibody
    • Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
    see all

Anti-eIF4EBP1 (phospho T45) antibody 图像

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47367. Left hand image without immunising peptide; right hand image with immunising peptide.


  • Predicted band size : 13 kDa

Anti-eIF4EBP1 (phospho T45) antibody (ab47367)参考文献

ab47367 has not yet been referenced specifically in any publications.

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