存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
纯度 Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/2000. Predicted molecular weight: 13 kDa.
应用说明 Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
功能 Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
序列相似性 Belongs to the eIF4E-binding protein family.
翻译后修饰 Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
Anti-eIF4EBP1 (phospho T37) antibody [EPR729(2)Y] 图像
Western blot - eIF4EBP1 (phospho T37) antibody [EPR729(2)Y] (ab75767)
All lanes : Anti-eIF4EBP1 (phospho T37) antibody [EPR729(2)Y] (ab75767) at 1/2000 dilution Lane 1 : 293 cell lysate, untreated Lane 2 : 293 cell lysate, treated with insulin Lysates/proteins at 10 µg per lane. Secondary HRP-conjugated goat anti-rabbit at 1/2000 dilution Predicted band size : 13 kDa Observed band size : 17 kDa ( why is the actual band size different from the predicted?)
Anti-eIF4EBP1 (phospho T37) antibody [EPR729(2)Y] (ab75767)参考文献
This product has been referenced in:
et al. The feedback loop of LITAF and BCL6 is involved in regulating apoptosis in B cell non-Hodgkin's-lymphoma. Oncotarget N/A:N/A (2016).
Read more (PubMed: 27764808) »
et al. Differentiation of glutamatergic neurons from mouse embryonic stem cells requires raptor S6K signaling. Stem Cell Res 11:1117-1128 (2013).
Read more (PubMed: 23988668) »
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for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"