RabMAb

Anti-eIF4EBP1 (phospho T37)抗体[EPR729(2)Y] (ab75767)

概述

  • 产品名称Anti-eIF4EBP1 (phospho T37)抗体[EPR729(2)Y]
    参阅全部 eIF4EBP1 一抗
  • 描述
    兔单克隆抗体[EPR729(2)Y] to eIF4EBP1 (phospho T37)
  • 特异性Detects eIF4EBP1 only when phosphorylated at threonine 37.
  • 经测试应用适用于: WBmore details
    不适用于: Flow Cyt,ICC/IF,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A phospho-peptide corresponding to residues surrounding threonine 37 of eIF4EBP1.

  • 阳性对照
    • 293 cells lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab75767 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Predicted molecular weight: 13 kDa.
  • 应用说明Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • 靶标

    • 功能Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
    • 序列相似性Belongs to the eIF4E-binding protein family.
    • 翻译后修饰Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 4E-BP1 antibody
      • 4EBP1 antibody
      • 4EBP1_HUMAN antibody
      • BP 1 antibody
      • eIF4E binding protein 1 antibody
      • eIF4E-binding protein 1 antibody
      • Eif4ebp1 antibody
      • Eukaryotic translation initiation factor 4E-binding protein 1 antibody
      • PHAS-I antibody
      • PHASI antibody
      • Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
      see all

    Anti-eIF4EBP1 (phospho T37) antibody [EPR729(2)Y] 图像

    • All lanes : Anti-eIF4EBP1 (phospho T37) antibody [EPR729(2)Y] (ab75767) at 1/2000 dilution

      Lane 1 : 293 cell lysate, untreated
      Lane 2 : 293 cell lysate, treated with insulin

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit at 1/2000 dilution

      Predicted band size : 13 kDa
      Observed band size : 17 kDa (why is the actual band size different from the predicted?)

    Anti-eIF4EBP1 (phospho T37) antibody [EPR729(2)Y] (ab75767)参考文献

    This product has been referenced in:
    • Shi Y  et al. The feedback loop of LITAF and BCL6 is involved in regulating apoptosis in B cell non-Hodgkin's-lymphoma. Oncotarget N/A:N/A (2016). Read more (PubMed: 27764808) »
    • Chuang JH  et al. Differentiation of glutamatergic neurons from mouse embryonic stem cells requires raptor S6K signaling. Stem Cell Res 11:1117-1128 (2013). Mouse . Read more (PubMed: 23988668) »

    See all 2 Publications for this product

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