概述

  • 产品名称Anti-eIF4EBP1 (phospho T36)抗体
    参阅全部 eIF4EBP1 一抗
  • 描述
    兔多克隆抗体to eIF4EBP1 (phospho T36)
  • 特异性ab47365 detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 36. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites.
  • 经测试应用适用于: ICC/IF, WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    The antiserum was produced against synthesized phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 36 (S-T-TP-P-G).

  • 阳性对照
    • MDA-MB-435 cell extracts; Human breast carcinoma tissue

性能

应用

Our Abpromise guarantee covers the use of ab47365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 13 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.

靶标

  • 功能Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
  • 序列相似性Belongs to the eIF4E-binding protein family.
  • 翻译后修饰Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 4E-BP1 antibody
    • 4EBP1 antibody
    • 4EBP1_HUMAN antibody
    • BP 1 antibody
    • eIF4E binding protein 1 antibody
    • eIF4E-binding protein 1 antibody
    • Eif4ebp1 antibody
    • Eukaryotic translation initiation factor 4E-binding protein 1 antibody
    • PHAS-I antibody
    • PHASI antibody
    • Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
    see all

Anti-eIF4EBP1 (phospho T36) antibody 图像

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47365. Left image without immunising peptide treatment; right image with immunising peptide treatment.
  • All lanes : Anti-eIF4EBP1 (phospho T36) antibody (ab47365)

    Lane 1 : MDA-MB-435 cells, treated with EGF (200 ng/ml, 30min)
    Lane 2 : MDA-MB-435 cells, untreated


    Predicted band size : 13 kDa
  • ICC/IF image of ab47365 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47365, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-eIF4EBP1 (phospho T36) antibody (ab47365)参考文献

This product has been referenced in:
  • Wang S  et al. Predictive value of phosphorylated mammalian target of rapamycin for disease-free survival in breast cancer patients receiving neoadjuvant chemotherapy. Oncol Lett 8:2642-2648 (2014). Read more (PubMed: 25364442) »

See 1 Publication for this product

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