概述

  • 产品名称
    Anti-eIF4EBP1抗体[Y330]
    参阅全部 eIF4EBP1 一抗
  • 描述
    兔单克隆抗体[Y330] to eIF4EBP1
  • 特异性
    ab32130 recognises 4E-BP1 (eIF4E-binding protein) also known as PHAS.
  • 经测试应用
    适用于: WB, IHC-P, Flow Cyt, IPmore details
    不适用于: ICC
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C-term of human4E-BP1

  • 阳性对照
    • K562 cell lysate and human prostate carcinoma
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab32130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 15-20 kDa (predicted molecular weight: 12 kDa).
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.
  • 应用说明
    Is unsuitable for ICC.
  • 靶标

    • 功能
      Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
    • 序列相似性
      Belongs to the eIF4E-binding protein family.
    • 翻译后修饰
      Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 4E-BP1 antibody
      • 4EBP1 antibody
      • 4EBP1_HUMAN antibody
      • BP 1 antibody
      • eIF4E binding protein 1 antibody
      • eIF4E-binding protein 1 antibody
      • Eif4ebp1 antibody
      • Eukaryotic translation initiation factor 4E-binding protein 1 antibody
      • PHAS-I antibody
      • PHASI antibody
      • Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
      see all

    Anti-eIF4EBP1 antibody [Y330] 图像



    • Predicted band size : 12 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: eIF4EBP1 knockout HAP1 cell lysate (20 µg)
      Lane 3: Hek293 cell lysate (20 µg)
      Lane 4: K562 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab32130 observed at 20 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab32130 was shown to specifically react with eIF4EBP1 when eIF4EBP1 knockout samples were used. Wild-type and eIF4EBP1 knockout samples were subjected to SDS-PAGE. ab32130 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • Anti-eIF4EBP1 antibody [Y330] (ab32130) at 1/1000 dilution + K562 cell lysate

      Predicted band size : 12 kDa
      Observed band size : 18 kDa (why is the actual band size different from the predicted?)
    • ab32130, staining human prostate carcinoma by immunohistochemistry, Paraffin embedded tissue

    Anti-eIF4EBP1 antibody [Y330] (ab32130)参考文献

    This product has been referenced in:
    • Lu Q  et al. Akt inhibition attenuates rasfonin-induced autophagy and apoptosis through the glycolytic pathway in renal cancer cells. Cell Death Dis 6:e2005 (2015). Human . Read more (PubMed: 26633711) »
    • Hu Y  et al. MiR-99a antitumor activity in human breast cancer cells through targeting of mTOR expression. PLoS One 9:e92099 (2014). WB ; Human . Read more (PubMed: 24637915) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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