The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 12 kDa).
功能Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
序列相似性Belongs to the eIF4E-binding protein family.
翻译后修饰Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
Immunohistochemical analysis of eIF4EBP1 expression in paraffin embedded human breast carcinoma tissue using 1/50 ab47428. Left: untreated sample. Right: sample treated with immunising peptide (negative control).
Anti-eIF4EBP1 antibody (ab47428)参考文献
has not yet been referenced specifically in any publications.