Anti-eIF4E (phospho S209)抗体(ab47605)

概述

  • 产品名称Anti-eIF4E (phospho S209)抗体
    参阅全部 eIF4E 一抗
  • 描述
    兔多克隆抗体to eIF4E (phospho S209)
  • 特异性This antibody is specific for elF4E, only when phosphorylated at serine 209.
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    A synthesized phosphopeptide derived from human elF4E around the phosphorylation site of serine 209 (S-G-SP-T-T)

  • 阳性对照
    • WB: Extracts from NIH/3T3 cells IHC: Human breast carcinoma tissue

性能

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应用

Our Abpromise guarantee covers the use of ab47605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
  • 序列相似性Belongs to the eukaryotic initiation factor 4E family.
  • 翻译后修饰Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AUTS19 antibody
    • CBP antibody
    • eIF 4E antibody
    • eIF 4F 25 kDa subunit antibody
    • EIF 4F antibody
    • eIF-4E antibody
    • eIF-4F 25 kDa subunit antibody
    • eIF4E antibody
    • EIF4E1 antibody
    • EIF4EL1 antibody
    • EIF4F antibody
    • Eukaryotic translation initiation factor 4 E antibody
    • Eukaryotic translation initiation factor 4E antibody
    • Eukaryotic translation initiation factor 4E like 1 antibody
    • IF4E_HUMAN antibody
    • Messanger RNA Cap Binding Protein eIF 4E antibody
    • MGC111573 antibody
    • mRNA cap binding protein antibody
    • mRNA cap-binding protein antibody
    see all

Anti-eIF4E (phospho S209) antibody 图像

  • All lanes : Anti-eIF4E (phospho S209) antibody (ab47605) at 1/500 dilution

    Lane 1 : Extracts from NIH/3T3 cells, treated with FBS. No peptide.
    Lane 2 : Extracts from NIH/3T3 cells, not treated with FBS. No peptide.

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 25 kDa
    Observed band size : 25 kDa
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47605 at a 1/50 dilution.

Anti-eIF4E (phospho S209) antibody (ab47605)参考文献

ab47605 has not yet been referenced specifically in any publications.

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