重组
RabMAb

Anti-eIF4E抗体[Y449] (ab33768)

概述

  • 产品名称
    Anti-eIF4E抗体[Y449]
    参阅全部 eIF4E 一抗
  • 描述
    兔单克隆抗体[Y449] to eIF4E
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IHC-P, Flow Cyt, IPmore details
    不适用于: ICC
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human eIF4E aa 150-250. The exact sequence is proprietary.

  • 阳性对照
    • WB: 293 cell lysate. IHC-P: Human breast carcinoma.
  • 常规说明

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab33768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/20000. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa).
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/40.
  • 应用说明
    Is unsuitable for ICC.
  • 靶标

    • 功能
      Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
    • 序列相似性
      Belongs to the eukaryotic initiation factor 4E family.
    • 翻译后修饰
      Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AUTS19 antibody
      • CBP antibody
      • eIF 4E antibody
      • eIF 4F 25 kDa subunit antibody
      • EIF 4F antibody
      • eIF-4E antibody
      • eIF-4F 25 kDa subunit antibody
      • eIF4E antibody
      • EIF4E1 antibody
      • EIF4EL1 antibody
      • EIF4F antibody
      • Eukaryotic translation initiation factor 4 E antibody
      • Eukaryotic translation initiation factor 4E antibody
      • Eukaryotic translation initiation factor 4E like 1 antibody
      • IF4E_HUMAN antibody
      • Messanger RNA Cap Binding Protein eIF 4E antibody
      • MGC111573 antibody
      • mRNA cap binding protein antibody
      • mRNA cap-binding protein antibody
      see all

    图片

    • All lanes : Anti-eIF4E antibody [Y449] (ab33768) at 1/500 dilution

      Lane 1 : C6 (Rat glioma) whole cell lysate
      Lane 2 : Raw264.7 (Mouse abelson murine leukemia virus-induced tumor) whole cell lysate
      Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
      Lane 4 : NIH/3T3 (Mouse embryo) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 25 kDa
      Observed band size: 28 kDa (why is the actual band size different from the predicted?)


      Exposure time: 3 minutes


      Blocking and diluting buffer: 5% NFDM/TBST.

    • Ab33768, at 1/250 dilution, staining eIF4E in paraffin embedded human breast carcinoma tissue sections by Immunohistochemistry.
    • Overlay histogram showing HEK293 cells stained with ab33768 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab33768, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Anti-eIF4E antibody [Y449] (ab33768) at 1/20000 dilution + 293 cell lysate

      Predicted band size: 25 kDa
      Observed band size: 30 kDa (why is the actual band size different from the predicted?)

    文献

    This product has been referenced in:
    • Oblinger JL  et al. Overexpression of eIF4F components in meningiomas and suppression of meningioma cell growth by inhibiting translation initiation. Exp Neurol 299:299-307 (2018). Read more (PubMed: 28610844) »

    See 1 Publication for this product

    客户评价及客户问答

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse embryonic stem cells (J1))
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    mouse embryonic stem cells (J1)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 Apr 10 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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