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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human eIF4E aa 1-100 (N terminal). The exact sequence is proprietary.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab33766 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/250 - 1/500.|
|WB||1/500. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time - Lane 1: 160 seconds
Lane 2: 70 seconds
ab33766 immunoprecipitating eIF4E in HEK293 whole cell lysate. Cell lysate was incubated with primary antibody (1/150 in 5% NFDM/TBST).
For western blotting ab33766 (1/1000) was used to confirm successful immunoprecipation. ab131366 was used as the secondary antibody. Blocking buffer: 5% NFDM/TBST.
Lane 1 (input): 10µg HEK293 whole cell lysate.
Lane 2 (+): HEK293 whole cell lysate and ab33766.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab33766 in HEK293 whole cell lysate.
Observed MW: 30kDa.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"