Anti-eIF4E抗体(ab47482)

概述

  • 产品名称Anti-eIF4E抗体
    参阅全部 eIF4E 一抗
  • 描述
    兔多克隆抗体to eIF4E
  • 特异性ab47482 detects endogenous levels of total elF4E protein.
  • 经测试应用适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic non-phosphopeptide derived from human elF4E around the phosphorylation site of serine 209 (S-G-SP-T-T).

  • 阳性对照
    • Breast carcinoma tissue. NIH/3T3 cells extract.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab47482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 25 kDa.

靶标

  • 功能Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
  • 序列相似性Belongs to the eukaryotic initiation factor 4E family.
  • 翻译后修饰Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AUTS19 antibody
    • CBP antibody
    • eIF 4E antibody
    • eIF 4F 25 kDa subunit antibody
    • EIF 4F antibody
    • eIF-4E antibody
    • eIF-4F 25 kDa subunit antibody
    • eIF4E antibody
    • EIF4E1 antibody
    • EIF4EL1 antibody
    • EIF4F antibody
    • Eukaryotic translation initiation factor 4 E antibody
    • Eukaryotic translation initiation factor 4E antibody
    • Eukaryotic translation initiation factor 4E like 1 antibody
    • IF4E_HUMAN antibody
    • Messanger RNA Cap Binding Protein eIF 4E antibody
    • MGC111573 antibody
    • mRNA cap binding protein antibody
    • mRNA cap-binding protein antibody
    see all

Anti-eIF4E antibody 图像

  • Immunohistochemical analysis of elF4E expression on paraffin-embedded human breast carcinoma tissue using ab47482 at a 1/50 dilution. Right panel: the Ab was incubated with the immunising peptide.
  • All lanes : Anti-eIF4E antibody (ab47482) at 1/500 dilution

    Lane 1 : FBS treated NIH/3T3 cells extract
    Lane 2 : FBS treated NIH/3T3 cells extract with blocking peptide


    Predicted band size : 25 kDa
    Observed band size : 25 kDa

Anti-eIF4E antibody (ab47482)参考文献

ab47482 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Prostate cell lines)
Loading amount 30 µg
Specification Prostate cell lines
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Oct 23 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"