概述

  • 产品名称
  • 描述
    兔多克隆抗体to eIF4E
  • 特异性
    ab47482 detects endogenous levels of total elF4E protein.
  • 经测试应用
    适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic non-phosphopeptide derived from human elF4E around the phosphorylation site of serine 209 (S-G-SP-T-T).

  • 阳性对照
    • Breast carcinoma tissue. NIH/3T3 cells extract.

性能

应用

Our Abpromise guarantee covers the use of ab47482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 25 kDa.

靶标

  • 功能
    Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
  • 序列相似性
    Belongs to the eukaryotic initiation factor 4E family.
  • 翻译后修饰
    Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AUTS19 antibody
    • CBP antibody
    • eIF 4E antibody
    • eIF 4F 25 kDa subunit antibody
    • EIF 4F antibody
    • eIF-4E antibody
    • eIF-4F 25 kDa subunit antibody
    • eIF4E antibody
    • EIF4E1 antibody
    • EIF4EL1 antibody
    • EIF4F antibody
    • Eukaryotic translation initiation factor 4 E antibody
    • Eukaryotic translation initiation factor 4E antibody
    • Eukaryotic translation initiation factor 4E like 1 antibody
    • IF4E_HUMAN antibody
    • Messanger RNA Cap Binding Protein eIF 4E antibody
    • MGC111573 antibody
    • mRNA cap binding protein antibody
    • mRNA cap-binding protein antibody
    see all

Anti-eIF4E antibody 图像

  • Immunohistochemical analysis of elF4E expression on paraffin-embedded human breast carcinoma tissue using ab47482 at a 1/50 dilution. Right panel: the Ab was incubated with the immunising peptide.
  • All lanes : Anti-eIF4E antibody (ab47482) at 1/500 dilution

    Lane 1 : FBS treated NIH/3T3 cells extract
    Lane 2 : FBS treated NIH/3T3 cells extract with blocking peptide


    Predicted band size : 25 kDa
    Observed band size : 25 kDa

Anti-eIF4E antibody (ab47482)参考文献

This product has been referenced in:
  • Singh J  et al. Immunohistochemical expression levels of p53 and eIF4E markers in histologically negative surgical margins, and their association with the clinical outcome of patients with head and neck squamous cell carcinoma. Mol Clin Oncol 4:166-172 (2016). Read more (PubMed: 26893854) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Prostate cell lines)
Loading amount
30 µg
Specification
Prostate cell lines
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Oct 23 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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