The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/25000 - 1/50000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
1/100 - 1/250.
Is unsuitable for Flow Cyt,IHC-P or IP.
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Contains 1 RRM (RNA recognition motif) domain.
Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.