概述

  • 产品名称Anti-eIF4B抗体[EP2299Y]
    参阅全部 eIF4B 一抗
  • 描述
    兔单克隆抗体[EP2299Y] to eIF4B
  • 经测试应用适用于: ICC/IF, WB, IHC-P, Flow Cytmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues on the C-terminus of human eIF4B.

  • 阳性对照
    • HeLa cell lysate, human prostate adenocarcinoma tissue.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号EP2299Y
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab68474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/250.
WB 1/200000. Detects a band of approximately 90 kDa (predicted molecular weight: 69 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
    • 序列相似性Contains 1 RRM (RNA recognition motif) domain.
    • 翻译后修饰Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 2310046H11Rik antibody
      • AL024095 antibody
      • C85189 antibody
      • EIF 4B antibody
      • eIF-4B antibody
      • EIF4B antibody
      • Eukaryotic initiation factor 4B antibody
      • Eukaryotic translation initiation factor 4B antibody
      • IF4B_HUMAN antibody
      • PRO1843 antibody
      see all

    Anti-eIF4B antibody [EP2299Y] 图像

    • Anti-eIF4B antibody [EP2299Y] (ab68474) at 1/200000 dilution + HeLa cell lysate at 10 µg

      Secondary
      HRP conjugated Goat anti-rabbit. at 1/2000 dilution

      Predicted band size : 69 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    • Human prostate adenocarcinoma stained with ab68474 at 1/100 dilution.

    Anti-eIF4B antibody [EP2299Y] (ab68474)参考文献

    This product has been referenced in:
    • Albornoz A  et al. The stress granule component TIA-1 binds tick-borne encephalitis virus RNA and is recruited to perinuclear sites of viral replication to inhibit viral translation. J Virol 88:6611-22 (2014). ICC/IF ; Human . Read more (PubMed: 24696465) »

    See 1 Publication for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
    Sample Human Cell (U2OS, osteosarcoma)
    Specification U2OS, osteosarcoma
    Permeabilization Yes - Triton 0.1%, 5 min
    Fixative Paraformaldehyde
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    提交于 Feb 13 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"