Anti-eIF4B抗体(ab66780)

概述

  • 产品名称Anti-eIF4B抗体
    参阅全部 eIF4B 一抗
  • 描述
    兔多克隆抗体to eIF4B
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cat
  • 免疫原

    Synthetic peptide: PTSGGGKVAP AQPSEEGPGR KDENKVDGMN APKGQTGNSS RGPGDGGNRD, corresponding to a region within C terminal amino acids 505-554 of Human eIF4B

  • 阳性对照
    • Jurkat cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab66780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 69 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 功能Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
  • 序列相似性Contains 1 RRM (RNA recognition motif) domain.
  • 翻译后修饰Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2310046H11Rik antibody
    • AL024095 antibody
    • C85189 antibody
    • EIF 4B antibody
    • eIF-4B antibody
    • EIF4B antibody
    • Eukaryotic initiation factor 4B antibody
    • Eukaryotic translation initiation factor 4B antibody
    • IF4B_HUMAN antibody
    • PRO1843 antibody
    see all

Anti-eIF4B antibody 图像

  • Anti-eIF4B antibody (ab66780) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 69 kDa
    Observed band size : 63,65 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling eIF4B with ab66780 at 4-8µg/ml. Arrows indicate positively labelled myocardial cells. Magnification: 400X.

Anti-eIF4B antibody (ab66780)参考文献

ab66780 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for contacting us regarding our eIF4B antibodies. We sell these three particular antibodies because they have been derived from different areas of human eIF4B. Ab78916 is derived from human eIF4B around the phosphorylation site ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"