概述

  • 产品名称Anti-eIF4A3抗体
    参阅全部 eIF4A3 一抗
  • 描述
    兔多克隆抗体to eIF4A3
  • 经测试应用适用于: IHC-P, IPmore details
    不适用于: WB
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 1-50 of Human eIF4A3 (NP_055555.1).

  • 阳性对照
    • Hela whole cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab115022 was affinity purified using an epitope specific to eIF4A3 immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab115022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能ATP-dependent RNA helicase. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Constitutes at least part of the platform anchoring other EJC proteins to spliced mRNAs. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH/RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed.
    • 组织特异性Ubiquitously expressed.
    • 序列相似性Belongs to the DEAD box helicase family. eIF4A subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • 细胞定位Nucleus. Nucleus speckle. Cytoplasm. Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Detected in dendritic layer as well as the nuclear and cytoplasmic (somatic) compartments of neurons. Colocalizes with STAU1 and FMR1 in dendrites.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ATP-dependent RNA helicase DDX48 antibody
      • ATP-dependent RNA helicase eIF4A-3 antibody
      • DDX48 antibody
      • DEAD box protein 48 antibody
      • eIF-4A-III antibody
      • eIF4A-III antibody
      • EIF4A3 antibody
      • eIF4AIII antibody
      • Eukaryotic initiation factor 4A-III antibody
      • Eukaryotic initiation factor 4A-like NUK-34 antibody
      • Eukaryotic translation initiation factor 4A isoform 3 antibody
      • hNMP 265 antibody
      • IF4A3_HUMAN antibody
      • NMP 265 antibody
      • NMP265 antibody
      • Nuclear matrix protein 265 antibody
      • NUK34 antibody
      see all

    Anti-eIF4A3 antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal carcinoma tissue labelling eIF4A3 with ab115022 at 1/1000 (1µg/ml). Detection: DAB.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling eIF4A3 with ab155022 at 1/5000 (0.2µg/ml). Detection: DAB.
    • Detection of eIF4A3 by Western Blot of Immunprecipitate.
      Anti-eIF4A3, at 0.4 µg/ml, staining eIF4A3 Lin Hea whole cell lysate immunoprecipitated using ab115022 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 30 seconds.

    Anti-eIF4A3 antibody (ab115022)参考文献

    ab115022 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab115022.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"