概述

  • 产品名称
  • 描述
    兔多克隆抗体to eIF4A2
  • 经测试应用
    适用于: IHC-P, IP, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human eIF4A2.

    (Peptide available as ab32473.)

  • 阳性对照
    • Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Brain (Mouse) Tissue Lysate Testis (Mouse) Tissue Lysate Skeletal Muscle (Mouse) Tissue Lysate (ab29711) Spinal Cord (Mouse) Tissue Lysate Ovary (Mouse) Tissue Lysate PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate Brain (Rat) Tissue Lysate Heart (Rat) Tissue Lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab31218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
ICC/IF 1/200.

靶标

  • 功能
    ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
  • 序列相似性
    Belongs to the DEAD box helicase family. eIF4A subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP dependent RNA helicase eIF4A 2 antibody
    • ATP-dependent RNA helicase eIF4A-2 antibody
    • BM-010 antibody
    • DDX2B antibody
    • eIF 4A II antibody
    • eIF-4A-II antibody
    • EIF4A antibody
    • eIF4A II antibody
    • eIF4A-II antibody
    • EIF4A2 antibody
    • EIF4F antibody
    • Eukaryotic initiation factor 4A II antibody
    • Eukaryotic initiation factor 4A-II antibody
    • eukaryotic translation initiation factor 4A isoform 2 antibody
    • Eukaryotic translation initiation factor 4A2 antibody
    • IF4A2_HUMAN antibody
    • N-terminally processed antibody
    see all

Anti-eIF4A2 antibody 图像

  • Anti-eIF4A2 antibody (ab31218) at 1 µg/ml + Jurkat whole cell lysate (ab7899) at 20 µg

    Secondary
    IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
    Additional bands at : 52 kDa. We are unsure as to the identity of these extra bands.
  • Immunocytochemistry/ Immunofluorescence analysis of MDA-MB231 Cells labeling eIF4A2 with ab31218 at a dilution of 1/200. Cells were fixed with Paraformaldehyde and permeabilized with 0.2% Saponin. The blocking step was incubation with 5% BSA for 30 minutes at 22°C. An Alexa Flour® 488 conjugated donkey anti-rabbit was used as the secondary antibody.

    See Abreview

  • Image courtesy of Human Protein Atlas. ab31218 staining eIF4A2 in human duodenum, showing strong cytomplasmic staining in the villi and Brunner's gland cells. Paraffin embedded human duodenum tissue was incubated with ab31218 (1/400 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab31218 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org

  • Mineralocorticoid Receptor was immunoprecipitated using 0.5mg Mouse skeletal muscle whole tissue extract, 5µg of Rabbit polyclonal to Mineralocorticoid Receptor and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse skeletal muscle whole tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab31218.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 47kDa: Mineralocorticoid Receptor.
  • All lanes : Anti-eIF4A2 antibody (ab31218) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Testis (Mouse) Tissue Lysate
    Lane 3 : Mouse skeletal muscle tissue lysate - total protein (ab29711)
    Lane 4 : Spinal Cord (Mouse) Tissue Lysate
    Lane 5 : Ovary (Mouse) Tissue Lysate
    Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 7 : Brain (Rat) Tissue Lysate
    Lane 8 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
    Additional bands at : 51 kDa. We are unsure as to the identity of these extra bands.

Anti-eIF4A2 antibody (ab31218)参考文献

This product has been referenced in:
  • Galicia-Vázquez G  et al. Regulation of eukaryotic initiation factor 4AII by MyoD during murine myogenic cell differentiation. PLoS One 9:e87237 (2014). WB ; Mouse . Read more (PubMed: 24466343) »
  • Menalled LB  et al. Genetic Deletion of Transglutaminase 2 Does Not Rescue the Phenotypic Deficits Observed in R6/2 and zQ175 Mouse Models of Huntington's Disease. PLoS One 9:e99520 (2014). WB ; Mouse . Read more (PubMed: 24955833) »

See all 9 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cells (CCE))
Total protein in input
600000 cells
Immuno-precipitation step
Protein G
Specification
mouse embryonic stem cells (CCE)
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Verified customer

提交于 Jan 30 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse ES Cells (CCE))
Permeabilization
Yes - 0.25% TritonX-100 solution (dilute in PBS)
Specification
Mouse ES Cells (CCE)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative
Paraformaldehyde
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提交于 Jan 27 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cells (CCE))
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Treatment
shRNA knockdown
Specification
mouse embryonic stem cells (CCE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Jan 26 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (hek293, hela)
Loading amount
50 µg
Specification
hek293, hela
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Feb 25 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Loading amount
40 µg
Specification
Mouse embryonic fibroblasts
Gel Running Conditions
Reduced Denaturing (7.5)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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提交于 Feb 24 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (MDA-MB231 and Hela Cell lines)
Loading amount
50000 cells
Specification
MDA-MB231 and Hela Cell lines
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Jul 05 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (MDA-MB231 Cells)
Specification
MDA-MB231 Cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Saponin
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Verified customer

提交于 Jul 04 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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