概述

  • 产品名称Anti-eIF4A1抗体
    参阅全部 eIF4A1 一抗
  • 描述
    兔多克隆抗体to eIF4A1
  • 经测试应用适用于: WB, IP, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human, African Green Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human eIF4A1.

    (Peptide available as ab31796.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) NIH 3T3 (Mouse embryonic fibroblast cell line) MEF1 (Mouse embryonic fibroblast cell line) PC12 (Rat adrenal pheochromocytoma cell line) This antibody gave a positive signal in the following tissue lysates: Liver (Mouse) Pancreas (Mouse) Testis (Mouse)

性能

应用

Our Abpromise guarantee covers the use of ab31217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
IP Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
  • 序列相似性Belongs to the DEAD box helicase family. eIF4A subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATP-dependent RNA helicase eIF4A-1 antibody
    • DDX2 antibody
    • DDX2A antibody
    • eIF 4A I antibody
    • eIF-4A-I antibody
    • EIF4A antibody
    • eIF4A I antibody
    • eIF4A-I antibody
    • EIF4A1 antibody
    • Eukaryotic initiation factor 4A-I antibody
    • Eukaryotic initiation factor 4AI antibody
    • eukaryotic translation initiation factor 4A, isoform 1 antibody
    • Eukaryotic translation initiation factor 4A1 antibody
    • IF4A1_HUMAN antibody
    see all

Anti-eIF4A1 antibody 图像

  • All lanes : Anti-eIF4A1 antibody (ab31217) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A431 whole cell lysate (ab7909)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
  • All lanes : Anti-eIF4A1 antibody (ab31217) at 1 µg/ml

    Lane 1 : NIH 3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Liver (Mouse) Tissue Lysate - normal tissue
    Lane 4 : Mouse pancreas tissue lysate - total protein (ab29363)
    Lane 5 : Testis (Mouse) Tissue Lysate - normal tissue
    Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
  • eIF4A1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to eIF4A1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab31217.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 47kDa: eIF4A1
  • ICC/IF image of ab31217 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab31217, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used to quench autofluorescence.  5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • Image courtesy of Human Protein Atlas. ab31217 staining eIF4A1 in human stomach, showing strong cytoplasmic staining in the glandular cells. Paraffin embedded human stomach tissue was incubated with ab31217 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab31217 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org

Anti-eIF4A1 antibody (ab31217)参考文献

This product has been referenced in:
  • Galicia-Vázquez G  et al. Regulation of eukaryotic initiation factor 4AII by MyoD during murine myogenic cell differentiation. PLoS One 9:e87237 (2014). WB ; Mouse . Read more (PubMed: 24466343) »
  • Robert F  et al. Translation initiation factor eIF4F modifies the dexamethasone response in multiple myeloma. Proc Natl Acad Sci U S A 111:13421-6 (2014). Read more (PubMed: 25197055) »

See all 13 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse neuroblastoma N2A cells)
Loading amount 30 µg
Specification Mouse neuroblastoma N2A cells
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Jul 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLA)
Loading amount 20 µg
Specification HeLA
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Jun 02 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - other (SW480)
Total protein in input 30 µg
Specification SW480
Immuno-precipitation step Protein G
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提交于 May 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample African Green Monkey Cell lysate - whole cell (Vero Cells)
Loading amount 10 µg
Specification Vero Cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Oct 24 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Formaldehyde
Permeabilization Yes - 0.5% NP40
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
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提交于 Oct 23 2007

Thank you for your enquiry. Unfortunately it appears there is no significant homology between the immunogen and the seuence you provided. It is unlikely this antibody will cross-react with sea urchin. I hope this information helps, please do not...

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