概述
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产品名称Anti-EIF3S2抗体
参阅全部 EIF3S2 一抗 -
描述兔多克隆抗体to EIF3S2
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宿主Rabbit
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经测试应用适用于: ICC/IF, WBmore details
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种属反应性与反应: Human
预测可用于: Mouse, Rabbit, Cow
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human EIF3S2.
(Peptide available as ab41640.)
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阳性对照
- ab41641 gave a positive signal in the following human whole cell lysates: HeLa, Jurkat, A431, HEK293, HepG2, MCF-7 and SHSY-5Y.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab41641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). |
靶标
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功能Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
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序列相似性Belongs to the eIF-3 subunit I family.
Contains 5 WD repeats. -
翻译后修饰Phosphorylated by TGF-beta type II receptor.
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细胞定位Cytoplasm.
- Information by UniProt
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数据库链接
- Entrez Gene: 521747 Cow
- Entrez Gene: 8668 Human
- Entrez Gene: 54709 Mouse
- Entrez Gene: 100008959 Rabbit
- Omim: 603911 Human
- SwissProt: Q5E966 Cow
- SwissProt: Q13347 Human
- SwissProt: Q9QZD9 Mouse
see all -
别名
- eIF-3-beta antibody
- eIF3 beta antibody
- eIF3 p36 antibody
see all
图片
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Western blot - Anti-EIF3S2 antibody (ab41641)All lanes : Anti-EIF3S2 antibody (ab41641) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-EIF3S2 antibody (ab41641)ICC/IF image of ab41641 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab41641, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
文献
ab41641 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"