概述
- 产品名称Anti-EIF3S2抗体
参阅全部 EIF3S2 一抗 - 描述兔多克隆抗体to EIF3S2
- 经测试应用适用于: ICC/IF, WBmore details
- 种属反应性与反应: Human
预测可用于: Mouse, Rabbit, Cow
- 免疫原
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human EIF3S2.
(Peptide available as ab41640.)
- 阳性对照
- ab41641 gave a positive signal in the following human whole cell lysates: HeLa, Jurkat, A431, HEK293, HepG2, MCF-7 and SHSY-5Y.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- 存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - 纯度Immunogen affinity purified
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab41641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). |
靶标
- 功能Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
- 序列相似性Belongs to the eIF-3 subunit I family.
Contains 5 WD repeats. - 翻译后修饰Phosphorylated by TGF-beta type II receptor.
- 细胞定位Cytoplasm.
- Information by UniProt
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数据库链接
- Entrez Gene: 521747 Cow
- Entrez Gene: 8668 Human
- Entrez Gene: 54709 Mouse
- Entrez Gene: 100008959 Rabbit
- Omim: 603911 Human
- SwissProt: Q5E966 Cow
- SwissProt: Q13347 Human
- SwissProt: Q9QZD9 Mouse
see all -
别名
- eIF-3-beta antibody
- eIF3 beta antibody
- eIF3 p36 antibody
see all
Anti-EIF3S2 antibody 图像
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Western blot - EIF3S2 antibody (ab41641)All lanes : Anti-EIF3S2 antibody (ab41641) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 36 kDa
Observed band size : 36 kDa
Additional bands at : 72 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - EIF3S2 antibody (ab41641)ICC/IF image of ab41641 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab41641, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Anti-EIF3S2 antibody (ab41641)参考文献
ab41641 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"