Anti-EIF3S1抗体- Carboxyterminal end (ab71416)

概述

  • 产品名称Anti-EIF3S1抗体- Carboxyterminal end
    参阅全部 EIF3S1 一抗
  • 描述
    兔多克隆抗体to EIF3S1 - Carboxyterminal end
  • 经测试应用适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (Human) from the C-terminal region of human EIF3S1, conjugated to KLH.

  • 阳性对照
    • K562 cell line lysate.

性能

应用

Our Abpromise guarantee covers the use of ab71416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/10 - 1/50.
WB 1/50 - 1/100. Detects a band of approximately 36 kDa (predicted molecular weight: 29 kDa).
ELISA 1/1000.

靶标

  • 功能Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit binds directly within the mRNA entry channel of the 40S ribosome to the aminoacyl (A) site. It may regulate the interaction between the 43S PIC and mRNA.
  • 序列相似性Belongs to the eIF-3 subunit J family.
  • 翻译后修饰Phosphorylated. Phosphorylation is enhanced upon serum stimulation.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • eIF 3 alpha antibody
    • eIF-3-alpha antibody
    • eIF3 alpha antibody
    • eIF3 p35 antibody
    • eIF3j antibody
    • EIF3J_HUMAN antibody
    • EIF3S 1 antibody
    • EIF3S1 antibody
    • Eukaryotic translation initiation factor 3 subunit 1 antibody
    • Eukaryotic translation initiation factor 3 subunit J antibody
    • Eukaryotic translation initiation factor 3, subunit 1 (alpha, 35kD) antibody
    • Eukaryotic translation initiation factor 3, subunit 1 alpha, 35kD antibody
    see all

Anti-EIF3S1 antibody - Carboxyterminal end 图像

  • Anti-EIF3S1 antibody - Carboxyterminal end (ab71416) at 1/60 dilution + K562 cell line lysates at 35 µg

    Predicted band size : 29 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • ab71416 at 1/50 dilution staining EIF3S1 - Carboxyterminal end in human lung carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

Anti-EIF3S1 antibody - Carboxyterminal end (ab71416)参考文献

ab71416 has not yet been referenced specifically in any publications.

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