概述

  • 产品名称Anti-eIF3K抗体
    参阅全部 eIF3K 一抗
  • 描述
    兔多克隆抗体to eIF3K
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide designed within residues: SQLKVWMSKY GWSADESGQI FICSQEE SIKPKNIVE KIDFDSVSSI MASSQ, corresponding to C terminal amino acids 168-218 of Human eIF3K (NP_037366.1)

  • 阳性对照
    • Whole cell lysates from HeLa or 293T cells.

性能

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应用

Our Abpromise guarantee covers the use of ab85968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 25 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
  • 组织特异性Ubiquitous, with the highest levels of expression in brain, testis and kidney.
  • 序列相似性Belongs to the eIF-3 subunit K family.
  • 细胞定位Nucleus. Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ARG134 antibody
    • eIF 3 p25 antibody
    • eIF 3 p28 antibody
    • eIF-3 p25 antibody
    • eIF-3 p28 antibody
    • EIF3-p28 antibody
    • eif3k antibody
    • EIF3K_HUMAN antibody
    • EIF3S12 antibody
    • Eukaryotic translation initiation factor 3 subunit 12 antibody
    • Eukaryotic translation initiation factor 3 subunit K antibody
    • HSPC029 antibody
    • M9 antibody
    • MSTP001 antibody
    • Muscle specific gene M9 protein antibody
    • Muscle-specific gene M9 protein antibody
    • PLAC 24 antibody
    • PLAC-24 antibody
    • PLAC24 antibody
    • PRO1474 antibody
    • PTD001 antibody
    see all

Anti-eIF3K antibody 图像

  • All lanes : Anti-eIF3K antibody (ab85968) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 25 kDa


    Exposure time : 10 seconds
  • 1 mg of HeLa whole cell lysate was immunoprecipitated using 3µg ab85968. Subsequently, 20% of IP was loaded per lane and probed with ab85968 at 1µg/ml (lane 1) or with a control IgG (lane 2).
    Detection: chemiluminescence with exposure time of 3 seconds.

Anti-eIF3K antibody (ab85968)参考文献

ab85968 has not yet been referenced specifically in any publications.

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