- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
- 产品名称Anti-EIF3F抗体
参阅全部 EIF3F 一抗 - 描述兔多克隆抗体to EIF3F
- 经测试应用适用于: WB, IHC-P, ELISAmore details
- 种属反应性与反应: Human
预测可用于: Mouse
- 免疫原
Synthetic peptide derived from internal sequence of human EIF3F.
- 阳性对照
- Human brain tissue. Extracts from Jurkat cells and COLO205 cells.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- 存储溶液Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... - 纯度Immunogen affinity purified
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab74568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 38 kDa. | |
| IHC-P | 1/50 - 1/100. | |
| ELISA | 1/40000. |
靶标
- 功能Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling. - 序列相似性Belongs to the eIF-3 subunit F family.
Contains 1 MPN (JAB/Mov34) domain. - 结构域The MPN domain mediates deubiquitinating activity.
- 翻译后修饰Phosphorylation is enhanced upon serum stimulation. Phosphorylated during apoptosis by caspase-processed CDK11.
- 细胞定位Cytoplasm.
- Information by UniProt
-
数据库链接
- Entrez Gene: 8665 Human
- Entrez Gene: 66085 Mouse
- Omim: 603914 Human
- SwissProt: O00303 Human
- SwissProt: Q9DCH4 Mouse
- Unigene: 516023 Human
- Unigene: 182962 Mouse
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别名
- Deubiquitinating enzyme eIF3f antibody
- eIF 3 epsilon antibody
- eIF-3-epsilon antibody
see all
Anti-EIF3F antibody 图像
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Western blot - EIF3F antibody (ab74568)All lanes : Anti-EIF3F antibody (ab74568) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from COLO205 cells
Lane 3 : Extracts from COLO205 cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 38 kDa
Observed band size : 38 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - EIF3F antibody (ab74568)Immunohistochemistry analysis of EIF3F in paraffin-embedded human brain tissue using ab74568, at 1/50-1/100 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
Anti-EIF3F antibody (ab74568)参考文献
This product has been referenced in:
- Villa N et al. Human eukaryotic initiation factor 4G (eIF4G) protein binds to eIF3c, -d, and -e to promote mRNA recruitment to the ribosome. J Biol Chem 288:32932-40 (2013). WB ; Human . Read more (PubMed: 24092755) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"