概述

  • 产品名称Anti-eIF3e抗体
    参阅全部 eIF3e 一抗
  • 描述
    兔多克隆抗体to eIF3e
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Medaka fish
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 1-50 of Human eIF3e (NP_001559.1).

  • 阳性对照
    • HeLa, 293T and NIH3T3 whole cell lysates

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab114851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 52 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.
  • 组织特异性Ubiquitously expressed. Expressed at highest levels in appendix, lymph, pancreas, skeletal muscle, spleen and thymus.
  • 序列相似性Belongs to the eIF-3 subunit E family.
    Contains 1 PCI domain.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasm. Nucleus > PML body.
  • Information by UniProt
  • 数据库链接
  • 别名
    • eIF-3 p48 antibody
    • eIF3e antibody
    • EIF3E_HUMAN antibody
    • EIF3S6 antibody
    • eIFe antibody
    • Eukaryotic translation initiation factor 3 subunit 6 antibody
    • Eukaryotic translation initiation factor 3 subunit E antibody
    • eukaryotic translation initiation factor 3, subunit 6 (48kD) antibody
    • INT6 antibody
    • mammary tumor-associated protein INT6 antibody
    • murine mammary tumor integration site 6 (oncogene homolog) antibody
    • Viral integration site protein INT-6 homolog antibody
    see all

Anti-eIF3e antibody 图像

  • All lanes : Anti-eIF3e antibody (ab114851) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 52 kDa


    Exposure time : 3 minutes
  • Detection of eIF3e in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab114851 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.

Anti-eIF3e antibody (ab114851)参考文献

ab114851 has not yet been referenced specifically in any publications.

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