概述

  • 产品名称Anti-EIF3D抗体
    参阅全部 EIF3D 一抗
  • 描述
    兔多克隆抗体to EIF3D
  • 经测试应用适用于: WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 200 and 250 of human EIF3D (NP_003744.1).

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

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应用

Our Abpromise guarantee covers the use of ab85951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
    • 序列相似性Belongs to the eIF-3 subunit D family.
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • eIF 3 zeta antibody
      • eIF-3-zeta antibody
      • eIF3 p66 antibody
      • eIF3 zeta antibody
      • eIF3d antibody
      • EIF3D_HUMAN antibody
      • eIF3p66 antibody
      • EIF3S7 antibody
      • Eukaryotic translation initiation factor 3 subunit 7 antibody
      • Eukaryotic translation initiation factor 3 subunit 7 zeta 66/67kDa antibody
      • Eukaryotic translation initiation factor 3 subunit D antibody
      • MGC126526 antibody
      • MGC17258 antibody
      • OTTHUMP00000197900 antibody
      • OTTHUMP00000197901 antibody
      • Translation initiation factor eIF3 p66 subunit antibody
      see all

    Anti-EIF3D antibody 图像

    • All lanes : Anti-EIF3D antibody (ab85951) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 64 kDa
      Observed band size : 64 kDa
      Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 3 minutes

    Anti-EIF3D antibody (ab85951)参考文献

    ab85951 has not yet been referenced specifically in any publications.

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