Anti-eIF3B抗体(ab113218)

概述

  • 产品名称Anti-eIF3B抗体
    参阅全部 eIF3B 一抗
  • 描述
    兔多克隆抗体to eIF3B
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 100-150 of Human eIF3B (NP_003742.2)

  • 阳性对照
    • Human: breast carcinoma; colon carcinoma; non-small cell lung cancer; ovarian carcinoma; prostate carcinoma; stomach adenocarcinoma; testicular seminoma (FFPE sections). Mouse: colon carcinoma CT26; renal cell carcinoma; teratoma (FFPE sections).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab113218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.

靶标

  • 功能Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
  • 序列相似性Belongs to the eIF-3 subunit B family.
    Contains 1 RRM (RNA recognition motif) domain.
    Contains 5 WD repeats.
  • 结构域The RRM domain mediates interaction with EIF3J.
  • 翻译后修饰Phosphorylated. Phosphorylation is enhanced upon serum stimulation.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • eIF-3-eta antibody
    • eIF3 eta antibody
    • eIF3 p110 antibody
    • eIF3 p116 antibody
    • EIF3-ETA antibody
    • EIF3-P110 antibody
    • EIF3-P116 antibody
    • eIF3b antibody
    • EIF3B_HUMAN antibody
    • EIF3S9 antibody
    • EIF3S9, formerly antibody
    • Eukaryotic translation initiation factor 3, subunit B antibody
    • Eukaryotic translation initiation factor 3 subunit 9 antibody
    • eukaryotic translation initiation factor 3 subunit 9 eta antibody
    • Eukaryotic translation initiation factor 3 subunit B antibody
    • eukaryotic translation initiation factor 3, subunit 9 (eta, 116kD) antibody
    • eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa antibody
    • eukaryotic translation initiation factor 3, subunit 9, formerly antibody
    • hPrt1 antibody
    • Protein synthesis defective at 36 degrees celsius 1, S. cerevisiae, homolog of antibody
    • PRT1 antibody
    • Prt1 homolog antibody
    see all

Anti-eIF3B antibody 图像

  • ab113218 at a 1/100 dilution staining eIF3B in Human ovarian carcinoma by immunohistochemistry (FFPE section). Secondary antibody used was Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed DyLight® 594 Conjugated at a dilution of 1/100.
  • ab113218 at a 1/500 dilution staining eIF3B in Human ovarian carcinoma by immunohistochemistry (FFPE section).
  • ab113218 at a 1/100 dilution staining eIF3B in Mouse teratoma by immunohistochemistry (FFPE section).

Anti-eIF3B antibody (ab113218)参考文献

ab113218 has not yet been referenced specifically in any publications.

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Vielen Dank für Ihre Antwort. Hier ist eine Liste mit den Homologien der Immunogene zu Kaninchen der folgenden Antikörper: eIF3B ABCF1=ABC50 ab40799 100% ab89109 92% ab113218 45% ...

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