Anti-EIF2S1 (phospho S51)抗体(ab4837)

概述

  • 产品名称Anti-EIF2S1 (phospho S51)抗体
    参阅全部 EIF2S1 一抗
  • 描述
    兔多克隆抗体to EIF2S1 (phospho S51)
  • 经测试应用适用于: ICC/IF, WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Human, Saccharomyces cerevisiae, Mink (Mustela)
  • 免疫原

    Synthetic peptide (Human) derived from the region of human eIF-2 alpha that contains serine 51. This region is conserved among many species including rat, pig, cow, fruit fly, and yeast.

  • 阳性对照
    • 3T3-L1 adipocytes stimulated with Leukemia Inhibitory Factor (LIF).

性能

应用

Our Abpromise guarantee covers the use of ab4837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).
IHC-Fr 1/250. See Abreview.

靶标

  • 功能Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
  • 序列相似性Belongs to the eIF-2-alpha family.
    Contains 1 S1 motif domain.
  • 翻译后修饰Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation (PubMed:15207627, PubMed:18032499). Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation.
  • 细胞定位Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity).
  • Information by UniProt
  • 数据库链接
  • 别名
    • EIF 2 alpha antibody
    • EIF 2 antibody
    • EIF 2A antibody
    • EIF 2alpha antibody
    • eIF-2-alpha antibody
    • eIF-2A antibody
    • EIF-2alpha antibody
    • EIF2 alpha antibody
    • EIF2 antibody
    • EIF2A antibody
    • EIF2S1 antibody
    • Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa antibody
    • Eukaryotic translation initiation factor 2 subunit 1 alpha antibody
    • Eukaryotic translation initiation factor 2 subunit 1 antibody
    • Eukaryotic translation initiation factor 2 subunit alpha antibody
    • IF2A_HUMAN antibody
    see all

Anti-EIF2S1 (phospho S51) antibody 图像



  • Predicted band size : 36 kDa


    Peptide Competition:

    Extracts prepared from 3T3-L1 adipocytes were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF.

    Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab4837 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with:
    1) the phosphopeptide immunogen
    2) the non-phosphopeptide corresponding to the immunogen
    3) no peptide

    After washing, membranes were incubated with goat F(ab)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab4837 blocks the antibody signal, thereby demonstrating the specificity of the antibody.

  • ab4837 staining eIF2S1(phospho S51) in mouse skeletal muscle tissue sections (5 µm) by IHC-Fr (Frozen sections). Tissue samples were fixed with acetone (for 10 minutes at -20°C) and blocked with 2% BSA for 1 hour at 25°C. The sample was incubated with primary antibody (1/250 in PBS with 1% BSA) at 4°C for 9 hours. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG (1/500) was used as secondary antibody.

    See Abreview

  • ab4837 staining eIF2S1 in human 293ft cells by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.1% Triton X-100 and then blocked using 5% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/200 for 2 hours at 25°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/250 dilution. DAPI was used to stain the cell nuclei (blue).

    See Abreview

Anti-EIF2S1 (phospho S51) antibody (ab4837)参考文献

This product has been referenced in:
  • Smith HK  et al. Translational signalling, atrogenic and myogenic gene expression during unloading and reloading of skeletal muscle in myostatin-deficient mice. PLoS One 9:e94356 (2014). WB ; Mouse . Read more (PubMed: 24718581) »
  • Hung SS  et al. Repression of Global Protein Synthesis by Eif1a-Like Genes That Are Expressed Specifically in the Two-Cell Embryos and the Transient Zscan4-Positive State of Embryonic Stem Cells. DNA Res N/A:N/A (2013). Mouse . Read more (PubMed: 23649898) »

See all 6 Publications for this product

Product Wall

Application Western blot
Sample Pig Cell lysate - whole cell (endothelial cell)
Gel Running Conditions Non-reduced Denaturing (8% gel)
Loading amount 30 µg
Specification endothelial cell
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Jul 24 2015

Thank you for your enquiry regarding ab4837 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this antibody. After reading through the detailed protocol you kin...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Brain lysate)
Loading amount 50 µg
Specification Brain lysate
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 20 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293FT cells)
Loading amount 100000 cells
Specification 293FT cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 02 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (293FT cell line)
Specification 293FT cell line
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 26 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (skeletal muscle)
Specification skeletal muscle
Fixative Acetone
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 05 2010

Thank you for this information and clarification. I have changed the online datasheet to reflect the fact that the antibody is raised against eIF2 alpha. Thank you again for your assistance.

Thank you for your enquiry. Our designation comes directly from Swiss-Prot where the synonyms for the protein are as follows: Eukaryotic translation initiation factor 2 alpha subunit eIF-2-alpha EIF-2alpha EIF-2A I believe the datasheet is...

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Thank you for your enquiry. I contacted the originator of this antibody and was provided with the following information. There is some history in the literature regarding this particular sequence. Basically, the serine residue is at position 51 in t...

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Thank you for your enquiry. Here are a few thoughts to consider: 1) EIF2a is a protein involved in translation of mRNA messages into protein. As such, I would look for it in the cytoplasm. 2) If someone is looking at serine phosphorylation, I ...

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