Anti-EIF2S1抗体(ab115822)
Key features and details
- Rabbit polyclonal to EIF2S1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-EIF2S1抗体
参阅全部 EIF2S1 一抗 -
描述
兔多克隆抗体to EIF2S1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide corresponding to a region of Rat EIF2S1.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab115822于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa. |
靶标
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功能
Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. -
序列相似性
Belongs to the eIF-2-alpha family.
Contains 1 S1 motif domain. -
翻译后修饰
Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation (PubMed:15207627, PubMed:18032499). Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation. -
细胞定位
Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). - Information by UniProt
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数据库链接
- Entrez Gene: 13665 Mouse
- SwissProt: Q6ZWX6 Mouse
- Unigene: 196220 Mouse
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别名
- EIF 2 alpha antibody
- EIF 2 antibody
- EIF 2A antibody
see all
图片
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab115822 被引用在 3 文献中.
- Huang Y et al. Elucidation of the mechanism of NEFA-induced PERK-eIF2a signaling pathway regulation of lipid metabolism in bovine hepatocytes. J Steroid Biochem Mol Biol 211:105893 (2021). PubMed: 33819629
- Guo Y et al. CHOP Regulates Endoplasmic Reticulum Stress-Mediated Hepatoxicity Induced by Monocrotaline. Front Pharmacol 12:685895 (2021). PubMed: 34108882
- Huang Y et al. NEFA Promotes Autophagosome Formation through Modulating PERK Signaling Pathway in Bovine Hepatocytes. Animals (Basel) 11:N/A (2021). PubMed: 34944177