概述

  • 产品名称
  • 描述
    山羊多克隆抗体to eIF2B4
  • 宿主
    Goat
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Cow, Dog
  • 免疫原

    Synthetic peptide:

    C-RKDYGSKVSLFSH

    , corresponding to internal sequence amino acids 190-202 of Human eIF2B4 (NP_751945.2; NP_001029288.1; NP_056451.3).

  • 阳性对照
    • K562 lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab104941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 58 kDa).

靶标

图片

  • Anti-eIF2B4 antibody (ab104941) at 0.3 µg/ml + K562 lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 58 kDa
    Observed band size: 60 kDa (why is the actual band size different from the predicted?)


    Exposure time: 1 hour


    An additional band of unknown identity was also consistently observed at 26 kDa. This band was successfully blocked by incubation with the immunizing peptide.

实验方案

文献

ab104941 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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