Anti-eIF2B epsilon抗体(ab91565)

概述

  • 产品名称Anti-eIF2B epsilon抗体
    参阅全部 eIF2B epsilon 一抗
  • 描述
    兔多克隆抗体to eIF2B epsilon
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 400-450 of Human eIF2B epsilon (NP_003898.2).

  • 阳性对照
    • HeLa and 293T whole cell lysates

性能

应用

Our Abpromise guarantee covers the use of ab91565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 80 kDa.
IP Use at 5-15 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.
  • 疾病相关Defects in EIF2B5 are a cause of leukodystrophy with vanishing white matter (VWM) [MIM:603896]. VWM is a leukodystrophy that occurs mainly in children. Neurological signs include progressive cerebellar ataxia, spasticity, inconstant optic atrophy and relatively preserved mental abilities. The disease is chronic-progressive with, in most individuals, additional episodes of rapid deterioration following febrile infections or minor head trauma. While childhood onset is the most common form of the disorder, some severe forms are apparent at birth. A severe, early-onset form seen among the Cree and Chippewayan populations of Quebec and Manitoba is called Cree leukoencephalopathy. Milder forms may not become evident until adolescence or adulthood. Some females with milder forms of the disease who survive to adolescence exhibit ovarian dysfunction. This variant of the disorder is called ovarioleukodystrophy.
  • 序列相似性Belongs to the eIF-2B gamma/epsilon subunits family.
    Contains 1 W2 domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CACH antibody
    • CLE antibody
    • EI2BE_HUMAN antibody
    • EIF 2B antibody
    • eIF 2B GDP GTP exchange factor antibody
    • eIF 2B GDP GTP exchange factor subunit epsilon antibody
    • eIF-2B GDP-GTP exchange factor subunit epsilon antibody
    • EIF2B antibody
    • EIF2B5 antibody
    • EIF2BE antibody
    • EIF2Bepsilon antibody
    • Eukaryotic Translation Initiation Factor 2 Beta Epsilon antibody
    • Eukaryotic translation initiation factor 2B subunit 5 epsilon 82kDa antibody
    • Eukaryotic translation initiation factor 2B subunit 5 epsilon antibody
    • LVWM antibody
    • Translation initiation factor eIF 2B subunit epsilon antibody
    • Translation initiation factor eIF-2B subunit epsilon antibody
    see all

Anti-eIF2B epsilon antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling eIF2B epsilon with ab91565 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-eIF2B epsilon antibody (ab91565) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 5 µg
    Lane 4 : HeLa whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 80 kDa


    Exposure time : 30 seconds
  • Detection of eIF2B epsilon by Western Blot of Immunprecipitate.
    ab91565, at 1 µg/ml, staining eIF2B epsilon in HeLa whole cell lysate immunoprecipitated using ab91565 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

Anti-eIF2B epsilon antibody (ab91565)参考文献

ab91565 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - whole cell (lung cells)
Negative control IgG
Specification lung cells
Detection step Semiquantitative PCR
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
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提交于 Jul 28 2016

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